Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-alpha-Fetoprotein Purified

Anti-alpha-Fetoprotein Purified

Anti-alpha-Fetoprotein Purified
Regulatory status
RUO
Antigen
alpha-Fetoprotein
Clone
AFP-01
Format
Purified
Reactivity
Human
Application
ELISA, IP, WB
Variant
0.1 mg
11-259-C100
In stock
110.00 USD
0.025 mg
11-259-C025
Out of stock
55.00 USD
Variant
0.1 mg
11-261-C100
In stock
110.00 USD
0.1 mg
11-261-C100
In stock
55.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Application
ELISA, IP, WB
Application details
ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).
Western blotting: Positive material: Hep G2 human hepatocellular carcinoma cell line; both non-reducing and reducing conditions.
Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
Reactivity
Human
Immunogen
purified alpha-Fetoprotein
Other names
AFP, FETA, HPAFP
Concentration
1 mg/ml
Preparation
Purified by precipitation and chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-259 WB
Western blotting analysis of human alpha-Fetoprotein in Hep G2 human hepatocellular carcinoma cell line. Lane 1: immunostaining with anti-human alpha-Fetoprotein (AFP-01); reducing conditions Lane 2: immunostaining with anti-human alpha-Fetoprotein (AFP-01); non-reducing conditions

Product specific references:

Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28;111(1):67-73.
PubMed
Cavin LG, Venkatraman M, Factor VM, Kaur S, Schroeder I, Mercurio F, Beg AA, Thorgeirsson SS, Arsura M: Regulation of alpha-fetoprotein by nuclear factor-kappaB protects hepatocytes from tumor necrosis factor-alpha cytotoxicity during fetal liver development and hepatic oncogenesis. Cancer Res. 2004 Oct 1;64(19):7030-8.
PubMed
Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.
PubMed
Variant
0.1 mg
11-259-C100
In stock
110.00 USD
0.025 mg
11-259-C025
Out of stock
55.00 USD
Variant
0.1 mg
11-261-C100
In stock
110.00 USD
0.1 mg
11-261-C100
In stock
55.00 USD
Contact distributor
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