Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-alpha-Fetoprotein Purified

Anti-alpha-Fetoprotein Purified

Anti-alpha-Fetoprotein Purified
Regulatory status
RUO
Antigen
alpha-Fetoprotein
Clone
AFP-01
Format
Purified
Reactivity
Human
Application
ELISA, IP, WB, ICC
Variant
0.1 mg
11-259-C100
In stock
110.00 USD
0.025 mg
11-259-C025
Delivery 1 week
55.00 USD
Variant
0.1 mg
11-259-C100
In stock
110.00 USD
0.025 mg
11-259-C025
Delivery 1 week
55.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Application
ELISA, IP, WB, ICC
Application details
ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
Reactivity
Human
Immunogen
purified alpha-Fetoprotein
Concentration
1 mg/ml
Preparation
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
AFP, FETA, HPAFP
Antigen description
Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Entrez Gene ID 174
UniProt ID P02771
11-259_WB
Western blotting analysis of human alpha-fetoprotein using mouse monoclonal antibody AFP-01 on lysates of HepG2 cell line and Jurkat cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-alpha-fetoprotein followed by IRDye800-conjugated anti-mouse secondary antibody. Alpha-fetoprotein was detected at approximately 60 kDa.

Product specific references:

Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28;111(1):67-73.
PubMed
Cavin LG, Venkatraman M, Factor VM, Kaur S, Schroeder I, Mercurio F, Beg AA, Thorgeirsson SS, Arsura M: Regulation of alpha-fetoprotein by nuclear factor-kappaB protects hepatocytes from tumor necrosis factor-alpha cytotoxicity during fetal liver development and hepatic oncogenesis. Cancer Res. 2004 Oct 1;64(19):7030-8.
PubMed
Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.
PubMed
Green CJ, Charlton CA, Wang LM, Silva M, Morten KJ, Hodson L: The isolation of primary hepatocytes from human tissue: optimising the use of small non-encapsulated liver resection surplus. Cell Tissue Bank 2017 Dec;18(4):597-604.
PubMed
Colquhoun DR, Goldman LR, Cole RN, Gucek M, Mansharamani M, Witter FR, Apelberg BJ, Halden RU: Global screening of human cord blood proteomes for biomarkers of toxic exposure and effect. Environ Health Perspect. 2009 May;117(5):832-8.
PubMed
Wang Z, Bi B, Song H, Liu L, Zheng H, Wang S, Shen Z: Proliferation of human hepatocellular carcinoma cells from surgically resected specimens under conditionally reprogrammed culture. Mol Med Rep. 2019 Jun;19(6):4623-4630.
PubMed
Variant
0.1 mg
11-259-C100
In stock
110.00 USD
0.025 mg
11-259-C025
Delivery 1 week
55.00 USD
Variant
0.1 mg
11-259-C100
In stock
110.00 USD
0.025 mg
11-259-C025
Delivery 1 week
55.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product