Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-AGR3 Purified

Anti-AGR3 Purified

Anti-AGR3 Purified
Regulatory status
RUO
Antigen
AGR3
Clone
AGR3.1
Format
Purified
Reactivity
Human
Application
WB, IHC(F), IHC(P), ICC
Variant
0.1 mg
11-560-C100
In stock
240.00 USD
0.025 mg
11-560-C025
Delivery 1 week
120.00 USD
Variant
0.1 mg
11-560-C100
In stock
240.00 USD
0.025 mg
11-560-C025
Delivery 1 week
120.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa); a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.
Application
WB, IHC(F), IHC(P), ICC
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 1 μg/ml; positive tissue: breast cancer.
Immunohistochemistry (frozen sections): Recommended dilution: 1 μg/ml; positive tissue: breast cancer.
Western blotting: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Immunocytochemistry: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Reactivity
Human
Immunogen
Purified human AGR3 protein
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
AG3, HAG3
Antigen description
AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.
Entrez Gene ID 155465
UniProt ID Q8TD06
11-560_IHC
Immunohistochemistry staining of AGR3 with mouse monoclonal AGR3.1 in paraffin-embedded sections of breast carcinoma.
11-560 WB
Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.

Product specific references:

Obacz J, Brychtová V, Podhorec J, Fabian P, Dobeš P, Vojtěšek B, Hrstka R: Anterior gradient protein 3 is associated with less aggressive tumors and better outcome of breast cancer patients. Onco Targets Ther. 2015 Jun 24;8:1523-32.
PubMed
Variant
0.1 mg
11-560-C100
In stock
240.00 USD
0.025 mg
11-560-C025
Delivery 1 week
120.00 USD
Variant
0.1 mg
11-560-C100
In stock
240.00 USD
0.025 mg
11-560-C025
Delivery 1 week
120.00 USD
Contact distributor
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