Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-AGR2/AGR3 Purified

Anti-AGR2/AGR3 Purified

Regulatory status

RUO

Antigen

AGR2/AGR3

Clone

AGR3.4

Format

Purified

Reactivity

Human

Application

WB, IHC(P), ICC

Variant

0.1 mg

11-562-C100

In stock

264.00 USD

0.025 mg

11-562-C025

Delivery 1 week

132.00 USD

Variant

0.1 mg

11-562-C100

In stock

264.00 USD

0.025 mg

11-562-C025

Delivery 1 week

132.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 kDa); secreted cytoplasmic proteins which can serve as markers of carcinogenesis.

Application

WB, IHC(P), ICC

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; Positive tissue: human colon.
Western blotting: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Immunocytochemistry: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.

Reactivity

Human

Immunogen

Purified human AGR3 protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

AG2, AG3, HAG2, HAG3

Antigen description

AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.

Immunohistochemistry staining of human colon (paraffin sections) using anti-AGR2+AGR3 (clone AGR3.4). Commercially tested by LifeSpan BioSciences.

Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.