Anti-AGR2/AGR3 Purified

Anti-AGR2/AGR3 Purified
Regulatory status
RUO
Antigen
AGR2/AGR3
Clone
AGR3.4
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-562-C100
In stock
264.00 USD

0.025 mg
11-562-C025
Delivery 1 week
132.00 USD
Variant
0.1 mg
11-562-C100
In stock
264.00 USD

0.025 mg
11-562-C025
Delivery 1 week
132.00 USD
Product details
Description
Images
SDS download
Isotype
Mouse IgG1
Specificity
The antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 kDa); secreted cytoplasmic proteins which can serve as markers of carcinogenesis.
Application
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; Positive tissue: human colon.
Western blotting: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Immunocytochemistry: Recommended dilution: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Reactivity
Human
Immunogen
Purified human AGR3 protein
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
AG2, AG3, HAG2, HAG3
Antigen description
AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.
11-562_IHC
Immunohistochemistry staining of human colon (paraffin sections) using anti-AGR2+AGR3 (clone AGR3.4). Commercially tested by LifeSpan BioSciences.
11-562_WB_appl
Anti-Hu AGR2/AGR3 Purified (clone AGR3.4) works in WB application.

Western blotting analysis was performed on whole cell extracts (HOT lysis buffer) of MCF-7, HeLa, and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing SDS-loading buffer. Samples were resolved using 15% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody AGR3.4 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.

AGR2/AGR3 molecules were detected at ~ 14-18 kDa in MCF-7 cell line.
11-562 WB
Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.
Variant
0.1 mg
11-562-C100
In stock
264.00 USD

0.025 mg
11-562-C025
Delivery 1 week
132.00 USD
Variant
0.1 mg
11-562-C100
In stock
264.00 USD

0.025 mg
11-562-C025
Delivery 1 week
132.00 USD