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Exbio
—
Research products
—
Antibodies
—
Other intracellular antigens
—
Anti-ZAP70 Purified
Anti-ZAP70 Purified
Regulatory status
RUO
Antigen
ZAP70
Clone
PAb (430)
Format
Purified
Reactivity
Human
Application
WB
Variant
0.1 mg
11-430-C100
Out of stock
180.00 USD
0.025 mg
11-430-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
Product details
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody recognizes C-terminal part of human ZAP70 protein tyrosine kinase (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Application
WB
Application details
Western blotting: Recommended dilution: 1 μg/ml; positive control: JURKAT cell line, reducing conditions.
Reactivity
Human
Immunogen
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
Other names
ZAP-70, ADMIO2
Concentration
1 mg/ml
Preparation
Purified from rabbit serum by affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
General references:
Chan AC, Irving BA, Fraser JD, Weiss A: The zeta chain is associated with a tyrosine kinase and upon T-cell antigen receptor stimulation associates with ZAP-70, a 70-kDa tyrosine phosphoprotein. Proc Natl Acad Sci USA 88(20), 9166 (1991).
PubMed
Ishaq M, DeGray G, Natarajan V: Evidence for the involvement of tyrosine kinase ZAP 70 in nuclear retinoid receptor-dependent transactivation in T lymphocytes. J Biol Chem. 2005 Oct 7;280(40):34152-8.
PubMed
Schneider H, Smith X, Liu H, Bismuth G, Rudd CE: CTLA-4 disrupts ZAP70 microcluster formation with reduced T cell/APC dwell times and calcium mobilization. Eur J Immunol. 2008 Jan;38(1):40-7.
PubMed
Variant
0.1 mg
11-430-C100
Out of stock
180.00 USD
0.025 mg
11-430-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
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