Anti-ZAP70 Purified

Anti-ZAP70 Purified
Regulatory status
RUO
Antigen
ZAP70
Clone
ZAP-03
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-488-C100
In stock
150.00 USD

0.025 mg
11-488-C025
Delivery 1 week
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Application
Application details
Flow cytometry: Intracellular staining; recommended dilution: 2-5 μg/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line.
Western blotting: Recommended dilution: 0,5 μg/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line; negative control: RAMOS human Burkitt lymphoma cell line; reducing conditions, 10% separating gel.
Reactivity
Human
Immunogen
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ZAP-70, ADMIO2
Antigen description
The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Entrez Gene ID 7535
UniProt ID P43403
11-488 WB
Western blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line. Lane 1, 2, 3, 4: immunostaining with dilution range of anti-ZAP-70 (ZAP-03; 4 μg/ml (1), 2 μg/ml (2), 1 μg/ml (3), 0.5 μg/ml (4) Lane 5: immunostaining with anti-ZAP-70 comparative antibody Lane 6: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)
11-488 WB_2
Western blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line (1, 3) and RAMOS human Burkitt lymphoma cell line (2, 4); Lane 1, 2: immunostaining with anti-ZAP-70 (ZAP-03; 0.5 μg/ml) Lane 3, 4: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)

General references:

Chan AC, Irving BA, Fraser JD, Weiss A: The zeta chain is associated with a tyrosine kinase and upon T-cell antigen receptor stimulation associates with ZAP-70, a 70-kDa tyrosine phosphoprotein. Proc Natl Acad Sci USA 88(20), 9166 (1991).
PubMed
Ishaq M, DeGray G, Natarajan V: Evidence for the involvement of tyrosine kinase ZAP 70 in nuclear retinoid receptor-dependent transactivation in T lymphocytes. J Biol Chem. 2005 Oct 7;280(40):34152-8.
PubMed
Schneider H, Smith X, Liu H, Bismuth G, Rudd CE: CTLA-4 disrupts ZAP70 microcluster formation with reduced T cell/APC dwell times and calcium mobilization. Eur J Immunol. 2008 Jan;38(1):40-7.
PubMed

Product specific references:

Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem. 2011 Jun 3;286(22):19617-29.
PubMed
Variant
0.1 mg
11-488-C100
In stock
150.00 USD

0.025 mg
11-488-C025
Delivery 1 week
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD

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