Anti-ZAP70 Purified

Anti-ZAP70 Purified
Regulatory status
RUO
Antigen
ZAP70
Clone
ZAP-03
Format
Purified
Reactivity
Human
Application
FC, WB, ICC
Variant
0.1 mg
11-488-C100
In stock
150.00 USD

0.025 mg
11-488-C025
Out of stock
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Application
FC, WB, ICC
Reactivity
Human
Immunogen
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
Other names
ZAP-70, ADMIO2
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-488 WB
Western blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line. Lane 1, 2, 3, 4: immunostaining with dilution range of anti-ZAP-70 (ZAP-03; 4 μg/ml (1), 2 μg/ml (2), 1 μg/ml (3), 0.5 μg/ml (4) Lane 5: immunostaining with anti-ZAP-70 comparative antibody Lane 6: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)
11-488 WB_2
Western blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line (1, 3) and RAMOS human Burkitt lymphoma cell line (2, 4); Lane 1, 2: immunostaining with anti-ZAP-70 (ZAP-03; 0.5 μg/ml) Lane 3, 4: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)

General references:

Chan AC, Irving BA, Fraser JD, Weiss A: The zeta chain is associated with a tyrosine kinase and upon T-cell antigen receptor stimulation associates with ZAP-70, a 70-kDa tyrosine phosphoprotein. Proc Natl Acad Sci USA 88(20), 9166 (1991).
PubMed
Ishaq M, DeGray G, Natarajan V: Evidence for the involvement of tyrosine kinase ZAP 70 in nuclear retinoid receptor-dependent transactivation in T lymphocytes. J Biol Chem. 2005 Oct 7;280(40):34152-8.
PubMed
Schneider H, Smith X, Liu H, Bismuth G, Rudd CE: CTLA-4 disrupts ZAP70 microcluster formation with reduced T cell/APC dwell times and calcium mobilization. Eur J Immunol. 2008 Jan;38(1):40-7.
PubMed

Product specific references:

Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem. 2011 Jun 3;286(22):19617-29.
PubMed
Variant
0.1 mg
11-488-C100
In stock
150.00 USD

0.025 mg
11-488-C025
Out of stock
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD