Exbio — Research products — Antibodies — Other intracellular antigens — Anti-VCP Purified

Anti-VCP Purified

Anti-VCP Purified
Regulatory status
RUO
Antigen
VCP
Clone
Hs-14
Format
Purified
Reactivity
Mouse, Human
Application
WB, FC (QC tested), ICC
Variant
0.1 mg
11-300-C100
In stock
170.00 USD
0.025 mg
11-300-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-300-C100
In stock
170.00 USD
0.025 mg
11-300-C025
Delivery 1 week
85.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgM
Specificity
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
Application
WB, FC (QC tested), ICC
Application details
Immunocytochemistry: Recommended dilution: 10 μg/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
Flow cytometry: Intraacrosomal staining; recommended dilution: 3-12 µg/ml.
Reactivity
Mouse, Human
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Concentration
1 mg/ml
Preparation
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TERA, CDC48
Antigen description
VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Entrez Gene ID 7415
UniProt ID P55072
11-300_ICC
Immunocytochemistry analysis of VCP in acetone-permeabilized human sperms (normal spermiogram)using monoclonal antibody Hs-14 demonstrates its location to the acrosome.

General references:

Pavlásek J, Peknicová J, Ulcová-Gallová Z, Nováková P, Reischig J, Micanová Z, Rokyta Z: Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction. Ceska Gynekol. 2004 Jul;69(4):306-11.
PubMed
Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.
PubMed

Product specific references:

Tepla O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).
Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32. Czech.
PubMed
Peknicova J, Chladek D, Hozak P: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol. 2005 Jan;53(1):42-9.
PubMed
Variant
0.1 mg
11-300-C100
In stock
170.00 USD
0.025 mg
11-300-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-300-C100
In stock
170.00 USD
0.025 mg
11-300-C025
Delivery 1 week
85.00 USD
Contact distributor
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