Exbio — Research products — Antibodies — Other intracellular antigens — Anti-VCP FITC

Anti-VCP FITC

Regulatory status

RUO

Antigen

VCP

Clone

Hs-14

Format

FITC

Reactivity

Mouse, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

50 tests

1F-300-T050

In stock

297.00 USD

Variant

50 tests

1F-300-T050

In stock

297.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.

Reactivity

Mouse, Human

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TERA, CDC48

Antigen description

VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Entrez Gene ID 7415

UniProt ID P55072

Anti-VCP FITC antibody (clone Hs-14) specificity verification by flow cytometry

Anti-VCP FITC antibody (concentration in sample 1 μg/ml, red-filled histogram) binds specifically to intracellular VCP in permeabilized human sperm cells (upper panel), but not to surface of non-permeabilized human sperm cells (lower panel). Level of non-specific binding was assessed using Mouse IgM isotype control FITC antibody (PFR-03) under same conditions (concentration in sample 1 μg/ml, black-dashed histogram).

General references:

Pavlásek J, Peknicová J, Ulcová-Gallová Z, Nováková P, Reischig J, Micanová Z, Rokyta Z: Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction. Ceska Gynekol. 2004 Jul;69(4):306-11.
PubMed

Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.
PubMed

Product specific references:

Tepla O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).

Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32. Czech.
PubMed

Peknicova J, Chladek D, Hozak P: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol. 2005 Jan;53(1):42-9.
PubMed