Exbio — Research products — Antibodies — Other intracellular antigens — Anti-TRIM Purified

Anti-TRIM Purified

Regulatory status

RUO

Antigen

TRIM

Clone

TRIM-04

Format

Purified

Reactivity

Human

Application

FC(IC), IP, WB

Variant

0.1 mg

11-344-C100

In stock

160.00 USD

0.025 mg

11-344-C025

Delivery 1 week

80.00 USD

Variant

0.1 mg

11-344-C100

In stock

160.00 USD

0.025 mg

11-344-C025

Delivery 1 week

80.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.

Application

FC(IC), IP, WB

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining.
Western blotting: Non-reducing conditions.

Reactivity

Human

Negative species

Mouse

Immunogen

Recombinant intracellular domain (aa 29-186) of human TRIM.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TRAT, TCRIM

Antigen description

TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.

Entrez Gene ID 50852

UniProt ID Q6PIZ9

Western blotting analysis of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb TRIM-04 and goat anti-mouse IgG (H+L)-HRP conjugate.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 positive TRIM positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 μg/ml, GAM APC).

General references:

Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed

Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49.
PubMed

Huynh T, Wurch A, Bruyns E, Korinek V, Schraven B, Eichmann K: Developmentally regulated expression of the transmembrane adaptor protein trim in fetal and adult T cells. Scand J Immunol. 2001 Jul-Aug;54(1-2):146-54.
PubMed

Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
PubMed

Product specific references:

Kirchgessner H, Dietrich J, Scherer J, Isomaki P, Korinek V, Hilgert I, Bruyns E, Leo A, Cope AP, Schraven B.: The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 2001 Jun 4;193(11):1269-84.
PubMed