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Exbio
—
Research products
—
Antibodies
—
Other intracellular antigens
—
Anti-TRIM PE
Anti-TRIM PE
Regulatory status
RUO
Antigen
TRIM
Clone
TRIM-04
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-344-C100
In stock
304.00 USD
0.025 mg
1P-344-C025
Delivery 1 week
152.00 USD
Variant
0.1 mg
1P-344-C100
In stock
304.00 USD
0.025 mg
1P-344-C025
Delivery 1 week
152.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG2a
Specificity
The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
Reactivity
Human
Negative species
Mouse
Immunogen
Recombinant intracellular domain (aa 29-186) of human TRIM.
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TRAT, TCRIM
Antigen description
TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
Entrez Gene ID
50852
UniProt ID
Q6PIZ9
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-TRIM (TRIM-04) PE antibody (concentration in sample 1 μg/ml).
Flow cytometry multicolor surface staining of human lymphocytes using anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-TRIM (TRIM-04) PE antibody (concentration in sample 1 μg/ml).
Comparison of anti-TRIM (TRIM-04) PE antibody nonspecific intracellular staining (red) and mouse IgG2a Isotype control (MOPC-173) PE antibody nonspecific intracellular staining (concentration in sample 5 μg/ml, same as anti-TRIM PE concentration, blue) co-stained by anti-mouse CD3 (145-2C11) APC antibody (concentration in sample 1,2 μg/ml) in flow cytometry analysis of murine splenocyte suspension.
Clone TRIM-04 is not specific for murine TRIM.
Separation of TRIM positive CD3 positive human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-TRIM (TRIM-04) PE antibody (concentration in sample 1 μg/ml).
Comparison of anti-TRIM (TRIM-04) PE antibody nonspecific staining (blue-filled) and mouse IgG2a Isotype control (MOPC-173) PE antibody nonspecific staining (concentration in sample 5 μg/ml, same as anti-TRIM PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of murine splenocyte suspension.
Clone TRIM-04 is not specific for murine TRIM.
General references:
Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed
Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49.
PubMed
Huynh T, Wurch A, Bruyns E, Korinek V, Schraven B, Eichmann K: Developmentally regulated expression of the transmembrane adaptor protein trim in fetal and adult T cells. Scand J Immunol. 2001 Jul-Aug;54(1-2):146-54.
PubMed
Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
PubMed
Product specific references:
Kirchgessner H, Dietrich J, Scherer J, Isomaki P, Korinek V, Hilgert I, Bruyns E, Leo A, Cope AP, Schraven B.: The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 2001 Jun 4;193(11):1269-84.
PubMed
Variant
0.1 mg
1P-344-C100
In stock
304.00 USD
0.025 mg
1P-344-C025
Delivery 1 week
152.00 USD
Variant
0.1 mg
1P-344-C100
In stock
304.00 USD
0.025 mg
1P-344-C025
Delivery 1 week
152.00 USD
Contact distributor
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