Exbio — Research products — Antibodies — Other intracellular antigens — Anti-TRIM PE

Anti-TRIM PE

Anti-TRIM PE
Regulatory status
RUO
Antigen
TRIM
Clone
TRIM-04
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-344-C100
In stock
304.00 USD
0.025 mg
1P-344-C025
Out of stock
152.00 USD
Variant
0.1 mg
11-261-C100
In stock
304.00 USD
0.1 mg
11-261-C100
In stock
152.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Application
FC (QC tested)
Application details
Flow cytometry: Intracellular staining. Recommended dilution: 1-5 μg/ml.
Reactivity
Human
Negative species
Mouse
Immunogen
Recombinant intracellular domain (aa 29-186) of human TRIM.
Other names
TRAT, TCRIM
Concentration
0.1 mg/ml
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1P-344 FC
Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-TRIM (TRIM-04) PE antibody (concentration in sample 3 μg/ml, red) or Mouse IgG2a Isotype Control (PPV-04) PE antibody (concentration in sample 3 μg/ml, blue).

General references:

Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed
Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49.
PubMed
Huynh T, Wurch A, Bruyns E, Korinek V, Schraven B, Eichmann K: Developmentally regulated expression of the transmembrane adaptor protein trim in fetal and adult T cells. Scand J Immunol. 2001 Jul-Aug;54(1-2):146-54.
PubMed
Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
PubMed

Product specific references:

Kirchgessner H, Dietrich J, Scherer J, Isomaki P, Korinek V, Hilgert I, Bruyns E, Leo A, Cope AP, Schraven B.: The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 2001 Jun 4;193(11):1269-84.
PubMed
Variant
0.1 mg
1P-344-C100
In stock
304.00 USD
0.025 mg
1P-344-C025
Out of stock
152.00 USD
Variant
0.1 mg
11-261-C100
In stock
304.00 USD
0.1 mg
11-261-C100
In stock
152.00 USD
Contact distributor
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