Exbio — Research products — Antibodies — Other intracellular antigens — Anti-TRIM PE

Anti-TRIM PE

Regulatory status

RUO

Antigen

TRIM

Clone

TRIM-04

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-344-C100

In stock

334.40 USD

0.025 mg

1P-344-C025

Delivery 1 week

167.20 USD

Variant

0.1 mg

1P-344-C100

In stock

334.40 USD

0.025 mg

1P-344-C025

Delivery 1 week

167.20 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Reactivity

Human

Negative species

Mouse

Immunogen

Recombinant intracellular domain (aa 29-186) of human TRIM.

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TRAT, TCRIM

Antigen description

TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.

Entrez Gene ID 50852

UniProt ID Q6PIZ9

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-TRIM (TRIM-04) PE antibody (concentration in sample 1 μg/ml).

Flow cytometry multicolor surface staining of human lymphocytes using anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-TRIM (TRIM-04) PE antibody (concentration in sample 1 μg/ml).

Comparison of anti-TRIM (TRIM-04) PE antibody nonspecific intracellular staining (red) and mouse IgG2a Isotype control (MOPC-173) PE antibody nonspecific intracellular staining (concentration in sample 5 μg/ml, same as anti-TRIM PE concentration, blue) co-stained by anti-mouse CD3 (145-2C11) APC antibody (concentration in sample 1,2 μg/ml) in flow cytometry analysis of murine splenocyte suspension.
Clone TRIM-04 is not specific for murine TRIM.

Separation of TRIM positive CD3 positive human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-TRIM (TRIM-04) PE antibody (concentration in sample 1 μg/ml).

Comparison of anti-TRIM (TRIM-04) PE antibody nonspecific staining (blue-filled) and mouse IgG2a Isotype control (MOPC-173) PE antibody nonspecific staining (concentration in sample 5 μg/ml, same as anti-TRIM PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of murine splenocyte suspension.
Clone TRIM-04 is not specific for murine TRIM.

General references:

Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed

Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49.
PubMed

Huynh T, Wurch A, Bruyns E, Korinek V, Schraven B, Eichmann K: Developmentally regulated expression of the transmembrane adaptor protein trim in fetal and adult T cells. Scand J Immunol. 2001 Jul-Aug;54(1-2):146-54.
PubMed

Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
PubMed

Product specific references:

Kirchgessner H, Dietrich J, Scherer J, Isomaki P, Korinek V, Hilgert I, Bruyns E, Leo A, Cope AP, Schraven B.: The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 2001 Jun 4;193(11):1269-84.
PubMed