Exbio — Research products — Antibodies — Other intracellular antigens — Anti-TFG Purified

Anti-TFG Purified

Regulatory status

RUO

Antigen

TFG

Clone

TFG-03

Format

Purified

Reactivity

Human

Application

IP, WB, ICC

Variant

0.1 mg

11-709-C100

In stock

198.00 USD

0.025 mg

11-709-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-709-C100

In stock

198.00 USD

0.025 mg

11-709-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody TFG-03 recognizes TFG, an approximately 50 kDa intracellular protein with regulatory functions.

Application

IP, WB, ICC

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human

Immunogen

Human recombinant protein Trx-his-NTFG

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TF6, TRKT3, SPG57

Antigen description

TFG (TRK-fused gene protein) is a regulatory protein with not fully understood function. Its defects are associated with various carcinomas, such as e.g. melanoma, thyroid papillary carcinoma, or glioma. TFG structure (multiple protein interaction motifs) indicated it can be an adaptor protein. It has been demonstrated TFG interacts with proteins modulating the NFkappaB pathway (TANK and NEMO). TNG enhances the effect of TNFalpha, TANK, TRAF2 and TRAF6 in inducing NFkappaB activity.

Entrez Gene ID 10342

UniProt ID Q92734

Western blotting analysis of TFG in Jurkat cell lysate using anti-TFG (TFG-03) purified.

General references:

Miranda C, Roccato E, Raho G, Pagliardini S, Pierotti MA, Greco A: The TFG protein, involved in oncogenic rearrangements, interacts with TANK and NEMO, two proteins involved in the NF-kappaB pathway. J Cell Physiol. 2006 Jul;208(1):154-60.
PubMed

Matsuda A, Suzuki Y, Honda G, Muramatsu S, Matsuzaki O, Nagano Y, Doi T, Shimotohno K, Harada T, Nishida E, Hayashi H, Sugano S: Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways. Oncogene. 2003 May 22;22(21):3307-18.
PubMed