Anti-STIM1 Purified

Anti-STIM1 Purified
Regulatory status
RUO
Antigen
STIM1
Clone
CDN3H4
Format
Purified
Reactivity
Rat, Human, Mouse
Variant
0.1 mg
11-497-C100
In stock
180.00 USD

0.025 mg
11-497-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.
Application details
Immunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml.
Western blotting: Recommended dilution: 1 μg/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions.
Reactivity
Rat, Human, Mouse
Immunogen
Synthetized peptide (C-terminal cytoplasmic part of STIM1).
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
GOK, TAM1, STRMK
Antigen description
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
Entrez Gene ID 6786
UniProt ID G0XQ39
11-497_ICC
Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor® 598; red). Nuclei were stained with DAPI (blue).
11-497 WB
Western blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)

General references:

Oh-Hora M, Yamashita M, Hogan PG, Sharma S, Lamperti E, Chung W, Prakriya M, Feske S, Rao A: Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance. Nat Immunol. 2008 Mar 9 [Epub ahead of print]
PubMed
Grigoriev I, Gouveia SM, van der Vaart B, Demmers J, Smyth JT, Honnappa S, Splinter D, Steinmetz MO, Putney JW Jr, Hoogenraad CC, Akhmanova A: STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER. Curr Biol. 2008 Feb 12;18(3):177-82.
PubMed
Hewavitharana T, Deng X, Soboloff J, Gill DL: Role of STIM and Orai proteins in the store-operated calcium signaling pathway. Cell Calcium. 2007 Aug;42(2):173-82.
PubMed
Cai X: Molecular evolution and functional divergence of the Ca2+ sensor protein in store-operated Ca2+ entry: stromal interaction molecule. PLoS ONE. 2007 Jul 11;2(7):e609.
PubMed
Peel SE, Liu B, Hall IP: A key role for STIM1 in store operated calcium channel activation in airway smooth muscle. Respir Res. 2006 Sep 20;7:119.
PubMed
Soboloff J, Spassova MA, Tang XD, Hewavitharana T, Xu W, Gill DL: Orai1 and STIM reconstitute store-operated calcium channel function. J Biol Chem. 2006 Jul 28;281(30):20661-5.
PubMed
Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA: Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J. 2001 Aug 1;357(Pt 3):673-85.
PubMed
Feske S: ORAI1 and STIM1 deficiency in human and mice: roles of store-operated Ca2+ entry in the immune system and beyond. Immunol Rev. 2009 Sep;231(1):189-209.
PubMed

Product specific references:

Hájková Z, Bugajev V, Dráberová E, Vinopal S, Dráberová L, Janáček J, Dráber P, Dráber P: STIM1-directed reorganization of microtubules in activated mast cells. J Immunol. 2011 Jan 15;186(2):913-23.
PubMed
Variant
0.1 mg
11-497-C100
In stock
180.00 USD

0.025 mg
11-497-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD