Anti-STIM1 Purified

Anti-STIM1 Purified
Regulatory status
RUO
Antigen
STIM1
Clone
CDN3H4
Format
Purified
Reactivity
Rat, Human, Mouse
Application
IP, WB, IHC(P), ICC
Variant
0.1 mg
11-497-C100
In stock

0.025 mg
11-497-C025
In stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody CDN3H4 reacts with human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.
Application
IP, WB, IHC(P), ICC
Reactivity
Rat, Human, Mouse
Immunogen
Synthetized peptide (C-terminal cytoplasmic part of STIM1).
Other names
GOK, TAM1, STRMK
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-497 ICC
Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor® 598; red). Nuclei were stained with DAPI (blue).
11-497 WB
Western blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)

General references:

Oh-Hora M, Yamashita M, Hogan PG, Sharma S, Lamperti E, Chung W, Prakriya M, Feske S, Rao A: Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance. Nat Immunol. 2008 Mar 9 [Epub ahead of print]
PubMed
Grigoriev I, Gouveia SM, van der Vaart B, Demmers J, Smyth JT, Honnappa S, Splinter D, Steinmetz MO, Putney JW Jr, Hoogenraad CC, Akhmanova A: STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER. Curr Biol. 2008 Feb 12;18(3):177-82.
PubMed
Hewavitharana T, Deng X, Soboloff J, Gill DL: Role of STIM and Orai proteins in the store-operated calcium signaling pathway. Cell Calcium. 2007 Aug;42(2):173-82.
PubMed
Cai X: Molecular evolution and functional divergence of the Ca2+ sensor protein in store-operated Ca2+ entry: stromal interaction molecule. PLoS ONE. 2007 Jul 11;2(7):e609.
PubMed
Peel SE, Liu B, Hall IP: A key role for STIM1 in store operated calcium channel activation in airway smooth muscle. Respir Res. 2006 Sep 20;7:119.
PubMed
Soboloff J, Spassova MA, Tang XD, Hewavitharana T, Xu W, Gill DL: Orai1 and STIM reconstitute store-operated calcium channel function. J Biol Chem. 2006 Jul 28;281(30):20661-5.
PubMed
Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA: Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J. 2001 Aug 1;357(Pt 3):673-85.
PubMed
Feske S: ORAI1 and STIM1 deficiency in human and mice: roles of store-operated Ca2+ entry in the immune system and beyond. Immunol Rev. 2009 Sep;231(1):189-209.
PubMed

Product specific references:

Hájková Z, Bugajev V, Dráberová E, Vinopal S, Dráberová L, Janáček J, Dráber P, Dráber P: STIM1-directed reorganization of microtubules in activated mast cells. J Immunol. 2011 Jan 15;186(2):913-23.
PubMed
Variant
0.1 mg
11-497-C100
In stock

0.025 mg
11-497-C025
In stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock