Exbio — Research products — Antibodies — Other intracellular antigens — Anti-STIM1 Purified

Anti-STIM1 Purified

Regulatory status

RUO

Antigen

STIM1

Clone

CDN3H4

Format

Purified

Reactivity

Rat, Human, Mouse

Application

IP, WB, IHC(P), ICC

Variant

0.1 mg

11-497-C100

In stock

180.00 USD

0.025 mg

11-497-C025

Delivery 1 week

90.00 USD

Variant

0.1 mg

11-497-C100

In stock

180.00 USD

0.025 mg

11-497-C025

Delivery 1 week

90.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Application

IP, WB, IHC(P), ICC

Application details

Immunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml.
Western blotting: Recommended dilution: 1 μg/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions.

Reactivity

Rat, Human, Mouse

Immunogen

Synthetized peptide (C-terminal cytoplasmic part of STIM1).

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

GOK, TAM1, STRMK

Antigen description

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

Entrez Gene ID 6786

UniProt ID G0XQ39

Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor® 598; red). Nuclei were stained with DAPI (blue).

Western blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)

General references:

Oh-Hora M, Yamashita M, Hogan PG, Sharma S, Lamperti E, Chung W, Prakriya M, Feske S, Rao A: Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance. Nat Immunol. 2008 Mar 9 [Epub ahead of print]
PubMed

Grigoriev I, Gouveia SM, van der Vaart B, Demmers J, Smyth JT, Honnappa S, Splinter D, Steinmetz MO, Putney JW Jr, Hoogenraad CC, Akhmanova A: STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER. Curr Biol. 2008 Feb 12;18(3):177-82.
PubMed

Hewavitharana T, Deng X, Soboloff J, Gill DL: Role of STIM and Orai proteins in the store-operated calcium signaling pathway. Cell Calcium. 2007 Aug;42(2):173-82.
PubMed

Cai X: Molecular evolution and functional divergence of the Ca2+ sensor protein in store-operated Ca2+ entry: stromal interaction molecule. PLoS ONE. 2007 Jul 11;2(7):e609.
PubMed

Peel SE, Liu B, Hall IP: A key role for STIM1 in store operated calcium channel activation in airway smooth muscle. Respir Res. 2006 Sep 20;7:119.
PubMed

Soboloff J, Spassova MA, Tang XD, Hewavitharana T, Xu W, Gill DL: Orai1 and STIM reconstitute store-operated calcium channel function. J Biol Chem. 2006 Jul 28;281(30):20661-5.
PubMed

Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA: Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J. 2001 Aug 1;357(Pt 3):673-85.
PubMed

Feske S: ORAI1 and STIM1 deficiency in human and mice: roles of store-operated Ca2+ entry in the immune system and beyond. Immunol Rev. 2009 Sep;231(1):189-209.
PubMed

Product specific references:

Hájková Z, Bugajev V, Dráberová E, Vinopal S, Dráberová L, Janáček J, Dráber P, Dráber P: STIM1-directed reorganization of microtubules in activated mast cells. J Immunol. 2011 Jan 15;186(2):913-23.
PubMed