Exbio — Research products — Antibodies — Other intracellular antigens — Anti-SOS Purified

Anti-SOS Purified

Regulatory status

RUO

Antigen

SOS

Clone

SOS-01

Format

Purified

Reactivity

Mouse, Human, Non-human primates

Application

WB, ICC

Variant

0.1 mg

11-522-C100

In stock

165.00 USD

0.025 mg

11-522-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-522-C100

In stock

165.00 USD

0.025 mg

11-522-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein. The epitope sequence is highly conserved and reactivity with multiple species is expected.

Application

WB, ICC

Application details

Western blotting: Recommended dilution: 1 μg/ml; positive control: HeLa human cervix carcinoma cell line, reducing conditions.

Reactivity

Mouse, Human, Non-human primates

Immunogen

Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SOS1, HGF, GGF1

Antigen description

The guanine nucleotide exchange factor Sos (son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.

Entrez Gene ID 6654

UniProt ID Q07889

Western blotting analysis (reducing conditions) of human Sos using anti-Sos (SOS-01). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line

Immunocytochemistry staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

General references:

Tian X, Feig LA: Basis for signaling specificity difference between Sos and Ras-GRF guanine nucleotide exchange factors. J Biol Chem. 2001 Dec 14;276(50):47248-56.
PubMed

Silver SJ, Chen F, Doyon L, Zink AW, Rebay I: New class of Son-of-sevenless (Sos) alleles highlights the complexities of Sos function. Genesis. 2004 Aug;39(4):263-72.
PubMed

Innocenti M, Tenca P, Frittoli E, Faretta M, Tocchetti A, Di Fiore PP, Scita G: Mechanisms through which Sos-1 coordinates the activation of Ras and Rac. J Cell Biol. 2002 Jan 7;156(1):125-36.
PubMed

Freedman TS, Sondermann H, Friedland GD, Kortemme T, Bar-Sagi D, Marqusee S, Kuriyan J: A Ras-induced conformational switch in the Ras activator Son of sevenless. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16692-7.
PubMed

Yang L, Bashaw GJ: Son of sevenless directly links the Robo receptor to rac activation to control axon repulsion at the midline. Neuron. 2006 Nov 22;52(4):595-607.
PubMed