Exbio — Research products — Antibodies — Other intracellular antigens — Anti-SIT Purified

Anti-SIT Purified

Regulatory status

RUO

Antigen

SIT

Clone

SIT-01

Format

Purified

Reactivity

Human

Application

FC, IP, WB

Variant

0.1 mg

11-345-C100

In stock

160.00 USD

0.025 mg

11-345-C025

Delivery 1 week

80.00 USD

Variant

0.1 mg

11-345-C100

In stock

160.00 USD

0.025 mg

11-345-C025

Delivery 1 week

80.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1

Specificity

The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.

Application

FC, IP, WB

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.

Reactivity

Human

Immunogen

Bacterially produced recombinant intracellular fragment of human SIT.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SIT1

Antigen description

SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.

Entrez Gene ID 27240

UniProt ID Q9Y3P8

Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-SIT monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. SIT was detected around 36 kDa.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/ml, GAM APC).

General references:

Hubener C, Mincheva A, Lichter P, Schraven B, Bruyns E: Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2-interacting transmembrane adaptor protein (SIT). Immunogenetics. 2001 May-Jun;53(4):337-41.
PubMed

Simeoni L, Posevitz V, Kolsch U, Meinert I, Bruyns E, Pfeffer K, Reinhold D, Schraven B: The transmembrane adapter protein SIT regulates thymic development and peripheral T-cell functions. Mol Cell Biol. 2005 Sep;25(17):7557-68.
PubMed

Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B: SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation. J Exp Med. 1999 Apr 19;189(8):1181-94.
PubMed

Product specific references:

Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed

Variant

0.1 mg

11-345-C100

In stock

160.00 USD

0.025 mg

11-345-C025

Delivery 1 week

80.00 USD

Variant

0.1 mg

11-345-C100