Exbio — Research products — Antibodies — Other intracellular antigens — Anti-SIT PE

Anti-SIT PE

Anti-SIT PE
Regulatory status
RUO
Antigen
SIT
Clone
SIT-01
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-345-C100
Out of stock
304.00 USD
0.025 mg
1P-345-C025
Out of stock
152.00 USD
Variant
0.1 mg
11-261-C100
In stock
304.00 USD
0.1 mg
11-261-C100
In stock
152.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.
Application
FC (QC tested)
Application details
Flow cytometry: Intracellular staining. Recommended dilution: 1-5 μg/ml.
Reactivity
Human
Immunogen
Bacterially produced recombinant intracellular fragment of human SIT.
Other names
SIT1
Concentration
0.1 mg/ml
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1P-345 FC
Flow cytometry analysis (intracellular staining) of human peripheral blood using anti-SIT (clone SIT-01) PE.

General references:

Hubener C, Mincheva A, Lichter P, Schraven B, Bruyns E: Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2-interacting transmembrane adaptor protein (SIT). Immunogenetics. 2001 May-Jun;53(4):337-41.
PubMed
Simeoni L, Posevitz V, Kolsch U, Meinert I, Bruyns E, Pfeffer K, Reinhold D, Schraven B: The transmembrane adapter protein SIT regulates thymic development and peripheral T-cell functions. Mol Cell Biol. 2005 Sep;25(17):7557-68.
PubMed
Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B: SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation. J Exp Med. 1999 Apr 19;189(8):1181-94.
PubMed

Product specific references:

Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed
Variant
0.1 mg
1P-345-C100
Out of stock
304.00 USD
0.025 mg
1P-345-C025
Out of stock
152.00 USD
Variant
0.1 mg
11-261-C100
In stock
304.00 USD
0.1 mg
11-261-C100
In stock
152.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product