Exbio — Research products — Antibodies — Other intracellular antigens — Anti-RPSA Biotin

Anti-RPSA Biotin

Regulatory status

RUO

Antigen

RPSA

Clone

RP-01

Format

Biotin

Reactivity

Mouse, Xenopus, Cow, Chicken, Human

Application

ELISA, IP, WB (verified), FC-IC (QC tested), IHC-P, ICC

Variant

0.1 mg

1B-829-C100

In stock

203.50 USD

Variant

0.1 mg

1B-829-C100

In stock

203.50 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody RP-01 recognizes ribosomal protein SA (RPSA), which is important for formation and stability of 40S ribosomal subunit, and is overexpressed in many carcinomas.

Application

ELISA, IP, WB (verified), FC-IC (QC tested), IHC-P, ICC

Application details

Flow cytometry: Recommended dilution: 2-10 μg/ml. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/ml; both reducing and non-reducing conditions.

Reactivity

Mouse, Xenopus, Cow, Chicken, Human

Immunogen

REPRLLVVTDPRADHQP

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Ribosomal protein SA, CCLBP, MRAP, LR1, LRP/LR

Antigen description

Ribosomal protein SA (RPSA) is a multi-functional protein, that is an important component of 40S ribosomal subunit, and binds to lamin. Higher expression of RPSA is characteristic for many carcinomas, and correlates with their invasivity and metastatic potential. It has also been described, that RPSA interacts with amyloid beta peptide during Alzheimer´s disease.

Entrez Gene ID 3921

UniProt ID P08865

Separation of HeLa cells stained using anti-RPSA (RP-01) purified antibody (concentration in sample 7.5 μg/ml, secondary stained using anti-biotin (1D4-C5) Alexa Fluor® 647 antibody (A6-944; 1µg/ml), red-filled) from HeLa cells unstained by primary antybody (secondary stained using anti-biotin Alexa Fluor® 647 antibody, black-dashed) in flow cytometry analysis (intracellular staining).

Anti-RPSA Biotin (clone RP-01) works in WB application under reducing conditions.

Western blotting analysis was performed on urea buffer extracts of Ramos, Raji, HeLa, Jurkat, and Huvec cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with biotinylated mouse IgG2a monoclonal antibody RP-01 (2 µg/ml), followed by IRDye 800CW Streptavidin (green). Multiplex fluorescent Western blot detection was performed.

RPSA was detected at ~37 kDa in all tested cell lines.