Exbio — Research products — Antibodies — Other intracellular antigens — Anti-PRR7 Purified

Anti-PRR7 Purified

Regulatory status

RUO

Antigen

PRR7

Clone

TRAP3/10

Format

Purified

Reactivity

Human, Mouse, Rat

Application

WB, ICC

Variant

0.1 mg

11-617-C100

In stock

242.00 USD

0.025 mg

11-617-C025

Delivery 1 week

121.00 USD

Variant

0.1 mg

11-617-C100

In stock

242.00 USD

0.025 mg

11-617-C025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.

Application

WB, ICC

Application details

Immunocytochemistry: Recommended dilution: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100.
Western blotting: Recommended dilution: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer).

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TRAP3

Antigen description

PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.

Entrez Gene ID 80758

UniProt ID Q8TB68

Immunocytochemistry staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).

Western blotting analysis of PRR7 using mouse monoclonal antibody TRAP3/10 on rat neuron lysate, and on HeLa transfectants overexpressing human PRR7, compared with non-transfected HeLa cells (negative control). Nitrocellulose membrane was probed with 1 µg/ml of mouse anti-PRR7 followed by a secondary antibody. PRR7 was detected around 35 kDa.

Western blotting detection of PRR7 / TRAP3 in murine brain lysate using the monoclonal antibody TRAP3/10.

Product specific references:

Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem. 2011 Jun 3;286(22):19617-29
PubMed