Exbio — Research products — Antibodies — Other intracellular antigens — Anti-PRKAR2A Purified

Anti-PRKAR2A Purified

Regulatory status

RUO

Antigen

PRKAR2A

Clone

Hs-36

Format

Purified

Reactivity

Human

Application

WB, ICC

Variant

0.1 mg

11-428-C100

Ask for delivery term HERE

176.00 USD

0.025 mg

11-428-C025

Delivery 1 week

88.00 USD

Variant

0.1 mg

11-428-C100

Ask for delivery term HERE

176.00 USD

0.025 mg

11-428-C025

Delivery 1 week

88.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), an intra-acrosomal protein.

Application

WB, ICC

Application details

Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential.

Reactivity

Human

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PKAR2A, PKR2, PKAR2, proteinkinase A regulatory 2

Antigen description

PRKAR2A (proteinkinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The PRKAR2A subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). In sperm, this antigen can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Entrez Gene ID 5576

UniProt ID P13861

Immunocytochemistry staining of normal human sperma with anti-PRKAR2A antibody (intracellular signal in acrosomes, green); DNA visualized by DAPI (blue).

General references:

Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.
PubMed

Product specific references:

Peknicova J, Chladek D, Hozak P: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol. 2005 Jan;53(1):42-9.
PubMed