Exbio — Research products — Antibodies — Other intracellular antigens — Anti-PPM1D Purified

Anti-PPM1D Purified

Regulatory status

RUO

Antigen

PPM1D

Clone

7E11/C5

Format

Purified

Reactivity

Mouse, Human

Application

ELISA, WB, IHC(P), ICC

Variant

0.1 mg

11-894-C100

In stock

154.00 USD

Variant

0.1 mg

11-894-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 7E11/C5 recognizes an epitope within the sequence FTNEDELYNLLTDSP of PPM1D, a protein phosphatase, which contributes to development of some carcinomas.

Application

ELISA, WB, IHC(P), ICC

Application details

Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Mouse, Human

Immunogen

Purified human PPM1D

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

WIP1, Protein phosphatase 1D, JDVS, WIP1, IDDGIP

Antigen description

PPM1D is a Mg2+/Mn2+ dependent protein phosphatase 1D with inducible expression in response to various types of environmental stress. This expression is p53-dependent, and subsequently PPM1D negatively regulates the p53-mediated transcription, thus it suppresses the apoptosis. PPM1D contributes to development of carcinomas, and seems to be a promissing therapeutic target. Amplification of PPM1D is associated with breast cancer.

Entrez Gene ID 8493

UniProt ID O15297

Western blotting analysis of human PPM1D using mouse monoclonal antibody 7E11/C5 on lysates of U2OS parental cells, expressing both natural (70 kDa, low expression) and C-terminally truncated version (55 kDa, high expression) of PPM1D, and on lysates of MCF7 cells (high PPM1D expression) and RPE cells (low PPM1D expression); reducing conditions.