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Exbio
—
Research products
—
Antibodies
—
Other intracellular antigens
—
Anti-PPM1D Alexa Fluor<sup>®</sup> 647
Anti-PPM1D Alexa Fluor
®
647
Regulatory status
RUO
Antigen
PPM1D
Clone
7E11/C5
Format
Alexa Fluor
®
647
Reactivity
Mouse, Human
Application
FC-IC (QC tested), IHC-P, ICC
Excitation laser
red (633 nm)
Variant
0.1 mg
A6-894-C100
In stock
242.00 USD
Variant
0.1 mg
A6-894-C100
In stock
242.00 USD
Contact distributor
Product details
Description
Images
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 7E11/C5 recognizes an epitope within the sequence FTNEDELYNLLTDSP of PPM1D, a protein phosphatase, which contributes to development of some carcinomas.
Application
FC-IC (QC tested), IHC-P, ICC
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Mouse, Human
Immunogen
Purified human PPM1D
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with Alexa Fluor
®
647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor
®
, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
WIP1, Protein phosphatase 1D, JDVS, WIP1, IDDGIP
Antigen description
PPM1D is a Mg2+/Mn2+ dependent protein phosphatase 1D with inducible expression in response to various types of environmental stress. This expression is p53-dependent, and subsequently PPM1D negatively regulates the p53-mediated transcription, thus it suppresses the apoptosis. PPM1D contributes to development of carcinomas, and seems to be a promissing therapeutic target. Amplification of PPM1D is associated with breast cancer.
Entrez Gene ID
8493
UniProt ID
O15297
Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-PPM1D (7E11/C5) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml).
Separation of MCF-7 cells stained using anti-PPM1D (7E11/C5) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml, red-filled) from MCF-7 cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml, same as PPM1D Alexa Fluor® 647, black-dashed) in flow cytometry analysis (intracellular staining).
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
A6-894-C100
In stock
242.00 USD
Variant
0.1 mg
A6-894-C100
In stock
242.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
Related products
A6-632-C100
Mouse IgG1 Isotype Control Alexa Fluor
®
647
-134%
Clone
MOPC-21
Reg. status
RUO
Application
FC, FC-IC, WB, IHC-F, IHC-P, ICC
0.1 mg
231.00 USD
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