Anti-Por Acrosin Purified

Anti-Por Acrosin Purified
Regulatory status
RUO
Antigen
Acrosin
Clone
ACR-2
Format
Purified
Reactivity
Pig
Application
WB, FC (QC tested), ICC
Variant
0.1 mg
11-260-C100
Out of stock
160.00 USD

0.025 mg
11-260-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Application
WB, FC (QC tested), ICC
Reactivity
Pig
Negative species
Dog, Cow, Human
Immunogen
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Other names
ACR, FBP53
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-260_ICC
Immunocytochemistry staining of capacitated boar sperm. Acrosome visualized using mouse monoclonal ACR-2 (purified; secondary antibody labeled with FITC, green), DNA stained with DAPI (blue).

Product specific references:

Peknicova J, Moos J. Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990 Sep-Oct;22(5):427-35.
PubMed
Moos J, Tesarik J, Leca G, Peknicova J. Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin. FEBS Lett. 1991 Dec 2;294(1-2):27-30.
PubMed
Moos J, Peknicova J, Tesarik J. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta. 1993 Apr 16;1176(3):199-207.
PubMed
Moos J, Peknicova J, Tesarik J. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod. 1993 Aug;49(2):408-15.
PubMed
Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M. Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro. Theriogenology. 2001 Jul 15;56(2):211-23.
PubMed
Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J: Effect of estrogens on boar sperm capacitation in vitro. Reprod Biol Endocrinol. 2010 Jul 13;8:87
PubMed
Děd L, Dostálová P, Žatecká E, Dorosh A, Komrsková K, Pěknicová J: Fluorescent analysis of boar sperm capacitation process in vitro. Reprod Biol Endocrinol. 2019 Dec 19;17(1):109.
PubMed
Variant
0.1 mg
11-260-C100
Out of stock
160.00 USD

0.025 mg
11-260-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD