Exbio — Research products — Antibodies — Other intracellular antigens — Anti-Por Acrosin Purified

Anti-Por Acrosin Purified

Regulatory status

RUO

Antigen

Acrosin

Clone

ACR-2

Format

Purified

Reactivity

Pig

Application

WB, FC (QC tested), ICC

Variant

0.1 mg

11-260-C100

In stock

176.00 USD

0.025 mg

11-260-C025

Delivery 1 week

88.00 USD

Variant

0.1 mg

11-260-C100

In stock

176.00 USD

0.025 mg

11-260-C025

Delivery 1 week

88.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.

Application

WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-12 µg/ml. Intracellular staining.
Immunocytochemistry: Membrane permeabilization (acetone) is essential.

Reactivity

Pig

Negative species

Dog, Cow, Human

Immunogen

Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ACR, FBP53

Antigen description

Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.

Entrez Gene ID 397098

UniProt ID P08001

Immunocytochemistry staining of capacitated boar sperm. Acrosome visualized using mouse monoclonal ACR-2 (purified; secondary antibody labeled with FITC, green), DNA stained with DAPI (blue).

Product specific references:

Peknicova J, Moos J. Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990 Sep-Oct;22(5):427-35.
PubMed

Moos J, Tesarik J, Leca G, Peknicova J. Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin. FEBS Lett. 1991 Dec 2;294(1-2):27-30.
PubMed

Moos J, Peknicova J, Tesarik J. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta. 1993 Apr 16;1176(3):199-207.
PubMed

Moos J, Peknicova J, Tesarik J. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod. 1993 Aug;49(2):408-15.
PubMed

Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M. Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro. Theriogenology. 2001 Jul 15;56(2):211-23.
PubMed

Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J: Effect of estrogens on boar sperm capacitation in vitro. Reprod Biol Endocrinol. 2010 Jul 13;8:87
PubMed

Děd L, Dostálová P, Žatecká E, Dorosh A, Komrsková K, Pěknicová J: Fluorescent analysis of boar sperm capacitation process in vitro. Reprod Biol Endocrinol. 2019 Dec 19;17(1):109.
PubMed