Anti-PKAc Purified

Anti-PKAc Purified
Regulatory status
RUO
Antigen
PKAc
Clone
6D2.1
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-523-C100
In stock
150.00 USD

0.025 mg
11-523-C025
Delivery 1 week
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 6D2.1 strongly reacts with human proteinkinase A (an intracellular antigen) catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
Application
Application details
Western blotting: Recommended dilution: 1 μg/ml; positive control: HeLa human cervix carcinoma cell line. To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).
Reactivity
Human
Immunogen
Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
PKACA, PPNAD4, PRKACA, Proteinkinase Ac
Antigen description
Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; proteinkinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit – PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.
Entrez Gene ID 5566
UniProt ID P17612
11-523 DB
Dot blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05; cat. no. 11-477-C100). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1: GST-Akt1 Lane 2: GST-Akt2 Lane 3: GST-Akt3 Lane 4: GST-PKAc alpha Lane 5: GST-PKAc beta Lane 6: GST-PKAc gamma Lane 7: GST-MEK 1 Lane 8: GST

General references:

Doucas V, Shi Y, Miyamoto S, West A, Verma I, Evans RM: Cytoplasmic catalytic subunit of protein kinase A mediates cross-repression by NF-kappa B and the glucocorticoid receptor. Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11893-8.
PubMed
Ferraris JD, Persaud P, Williams CK, Chen Y, Burg MB: cAMP-independent role of PKA in tonicity-induced transactivation of tonicity-responsive enhancer/ osmotic response element-binding protein. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16800-5.
PubMed
Komagiri Y, Kitamura N: Comparison of effects of PKA catalytic subunit on I(h) and calcium channel currents in rat dorsal root ganglion cells. Biomed Res. 2007 Aug;28(4):177-89.
PubMed
Kiger JA Jr, Eklund JL, Younger SH, O'Kane CJ: Transgenic inhibitors identify two roles for protein kinase A in Drosophila development. Genetics. 1999 May;152(1):281-90.
PubMed
Beebe SJ, Oyen O, Sandberg M, Frøysa A, Hansson V, Jahnsen T: Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase. Mol Endocrinol. 1990 Mar;4(3):465-75.
PubMed
Variant
0.1 mg
11-523-C100
In stock
150.00 USD

0.025 mg
11-523-C025
Delivery 1 week
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD