Exbio — Research products — Antibodies — Other intracellular antigens — Anti-PDE8A Purified

Anti-PDE8A Purified

Regulatory status

RUO

Antigen

PDE8A

Clone

EM-52

Format

Purified

Reactivity

Human

Application

Variant

0.1 mg

11-710-C100

In stock

198.00 USD

0.025 mg

11-710-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-710-C100

In stock

198.00 USD

0.025 mg

11-710-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody EM-52 recognizes PDE8a (phosphodiesterase 8 A), an approximately 93 kDa intracellular enzyme that hydrolyzes cAMP to 5'AMP.

Application

Application details

Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Human

Immunogen

Human PDE8a

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

HsT19550

Antigen description

PDE8a (high affinity cAMP-specific and IBMX-insensitive 3´,5´-cyclic phosphodiesterase 8 A) is a cyclic nucleotide phosphodiesterase, which hydrolyzes cAMP, an important regulator and mediator of multiple cellular responses to extracellular signals. It is expressed in a wide variety of tissues including testis, ovary, small intestine, and colon. It is not expressed e.g. in thymus. PDE8a is composed of C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Entrez Gene ID 5151

UniProt ID O60658

Western bloting analysis of human PDE8A using mouse monoclonal antibody EM-52 on lysates of HeLa cell line and TF-1 cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-PDE8A followed by Alexa Fluor® 790-conjugated goat-anti-mouse secondary antibody. A specific band was detected for PDE8A at approximately 95 kDa.

General references:

Wang H, Yan Z, Yang S, Cai J, Robinson H, Ke H: Kinetic and structural studies of phosphodiesterase-8A and implication on the inhibitor selectivity. Biochemistry. 2008 Dec 2;47(48):12760-8. doi: 10.1021/bi801487x.
PubMed

Brown KM, Day JP, Huston E, Zimmermann B, Hampel K, Christian F, Romano D, Terhzaz S, Lee LC, Willis MJ, Morton DB, Beavo JA, Shimizu-Albergine M, Davies SA, Kolch W, Houslay MD, Baillie GS: Phosphodiesterase-8A binds to and regulates Raf-1 kinase. Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):E1533-42
PubMed