Anti-PDE8A Purified

Anti-PDE8A Purified
Regulatory status
RUO
Antigen
PDE8A
Clone
EM-52
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-710-C100
In stock
180.00 USD

0.025 mg
11-710-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody EM-52 recognizes PDE8a (phosphodiesterase 8 A), an approximately 93 kDa intracellular enzyme that hydrolyzes cAMP to 5'AMP.
Application
Application details
Western blotting: Recommended dilution: 1-2 μg/ml.
Reactivity
Human
Immunogen
Human PDE8a
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
HsT19550
Antigen description
PDE8a (high affinity cAMP-specific and IBMX-insensitive 3´,5´-cyclic phosphodiesterase 8 A) is a cyclic nucleotide phosphodiesterase, which hydrolyzes cAMP, an important regulator and mediator of multiple cellular responses to extracellular signals. It is expressed in a wide variety of tissues including testis, ovary, small intestine, and colon. It is not expressed e.g. in thymus. PDE8a is composed of C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Entrez Gene ID 5151
UniProt ID O60658
11-710_WB
Western bloting analysis of human PDE8A using mouse monoclonal antibody EM-52 on lysates of HeLa cell line and TF-1 cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-PDE8A followed by Alexa Fluor® 790-conjugated goat-anti-mouse secondary antibody. A specific band was detected for PDE8A at approximately 95 kDa.

General references:

Wang H, Yan Z, Yang S, Cai J, Robinson H, Ke H: Kinetic and structural studies of phosphodiesterase-8A and implication on the inhibitor selectivity. Biochemistry. 2008 Dec 2;47(48):12760-8. doi: 10.1021/bi801487x.
PubMed
Brown KM, Day JP, Huston E, Zimmermann B, Hampel K, Christian F, Romano D, Terhzaz S, Lee LC, Willis MJ, Morton DB, Beavo JA, Shimizu-Albergine M, Davies SA, Kolch W, Houslay MD, Baillie GS: Phosphodiesterase-8A binds to and regulates Raf-1 kinase. Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):E1533-42
PubMed
Variant
0.1 mg
11-710-C100
In stock
180.00 USD

0.025 mg
11-710-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD