Exbio — Research products — Antibodies — Other intracellular antigens — Anti-PAG1 Purified

Anti-PAG1 Purified

Anti-PAG1 Purified
Regulatory status
RUO
Antigen
PAG1
Clone
PAG-C1
Format
Purified
Reactivity
Rat, Cow, Human, Mouse
Application
IP, WB, IHC-P
Variant
0.1 mg
11-346-C100
In stock
203.50 USD
0.025 mg
11-346-C025
In stock
101.75 USD
Variant
0.1 mg
11-346-C100
In stock
203.50 USD
0.025 mg
11-346-C025
In stock
101.75 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b
Specificity
The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Application
IP, WB, IHC-P
Application details
Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1.
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.
Reactivity
Rat, Cow, Human, Mouse
Immunogen
C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CBP, PAG
Antigen description
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Entrez Gene ID 94212
UniProt ID Q3U1F9
11-346 IP
Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human PAG (MEM-255; cat. no. 11-347-C100). Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-PAG (PAG-C1) Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody; cat. no. 11-409-C100)
11-346_IHC
Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in human appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.

Product specific references:

Brdicka T, Pavlistová D, Leo A, Bruyns E, Korínek V, Angelisová P, Scherer J, Shevchenko A, Hilgert I, Cerný J, Drbal K, Kuramitsu Y, Kornacker B, Horejsí V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604.
PubMed
Vang T, Abrahamsen H, Myklebust S, Horejsi V, Tasken K: Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. J Biol Chem. 2003 May 16;278(20):17597-600.
PubMed
Variant
0.1 mg
11-346-C100
In stock
203.50 USD
0.025 mg
11-346-C025
In stock
101.75 USD
Variant
0.1 mg
11-346-C100
In stock
203.50 USD
0.025 mg
11-346-C025
In stock
101.75 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product