Exbio — Research products — Antibodies — Other intracellular antigens — Anti-PAG1 Purified

Anti-PAG1 Purified

Regulatory status

RUO

Antigen

PAG1

Clone

PAb (409)

Format

Purified

Reactivity

Mouse, Human

Application

IP, WB, IHC(P)

Variant

0.1 mg

11-409-C100

In stock

198.00 USD

0.025 mg

11-409-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-409-C100

In stock

198.00 USD

0.025 mg

11-409-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Rabbit polyclonal

Specificity

The polyclonal antibody recognizes intracellular part of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Application

IP, WB, IHC(P)

Application details

Immunohistochemistry (paraffin sections): Positive tissue: tonsil, colon germinal center.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Mouse, Human

Immunogen

Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CBP, PAG

Antigen description

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Entrez Gene ID 94212

UniProt ID Q3U1F9

Western blotting analysis of human PAG using rabbit polyclonal antibody PAb (409) on lysates of Daudi and Ramos cell line under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-PAG monoclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. PAG was detected around 65 kDa.