Exbio — Research products — Antibodies — Other intracellular antigens — Anti-PAG1 Purified

Anti-PAG1 Purified

Regulatory status

RUO

Antigen

PAG1

Clone

PAG-C1

Format

Purified

Reactivity

Rat, Cow, Human, Mouse

Application

IP, WB, IHC(P)

Variant

0.1 mg

11-346-C100

In stock

180.00 USD

0.025 mg

11-346-C025

In stock

90.00 USD

Variant

0.1 mg

11-346-C100

In stock

180.00 USD

0.025 mg

11-346-C025

In stock

90.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Application

IP, WB, IHC(P)

Application details

Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1.
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.

Reactivity

Rat, Cow, Human, Mouse

Immunogen

C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CBP, PAG

Antigen description

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Entrez Gene ID 94212

UniProt ID Q3U1F9

Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human PAG (MEM-255; cat. no. 11-347-C100). Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-PAG (PAG-C1) Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody; cat. no. 11-409-C100)

Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in human appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.

Product specific references:

Brdicka T, Pavlistová D, Leo A, Bruyns E, Korínek V, Angelisová P, Scherer J, Shevchenko A, Hilgert I, Cerný J, Drbal K, Kuramitsu Y, Kornacker B, Horejsí V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604.
PubMed

Vang T, Abrahamsen H, Myklebust S, Horejsi V, Tasken K: Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. J Biol Chem. 2003 May 16;278(20):17597-600.
PubMed