Anti-OPAL1 Purified

Anti-OPAL1 Purified
Regulatory status
RUO
Antigen
OPAL1
Clone
OPAL1-01
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-699-C100
Ask for delivery term HERE
198.00 USD

0.025 mg
11-699-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
11-699-C100
Ask for delivery term HERE
198.00 USD

0.025 mg
11-699-C025
Delivery 1 week
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody OPAL1-01 recognizes an intracellular epitope of OPAL1 (outcome predictor in acute leukemia 1), a transmembrane adaptor protein expressed mainly by megacaryocytes and platelets.
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/ml, non-reducing conditions.
Reactivity
Human
Immunogen
Recombinant fragment of human OPAL1 (amino acids 152-342)
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
WBP1L
Antigen description
OPAL1 (outcome predictor in acute leukemia 1) is an almost uncharacterized transmembrane adaptor protein expressed mainly by megacaryocytes and platelets. Its expression is enhanced on TEL/AML leukemic cells. The function of OPAL1 is unknown, although the presence of a cytochrome c-like heme-binding site and a transmembrane domain suggested OPAL1 may be involved in the mitochondrial electron transport chain. Its originally reported prognostic impact appears to be treatment dependent.
Entrez Gene ID 54838
UniProt ID Q9NX94
11-699 FC
Flow cytometry analysis of OPAL-transfected HEK293 cells using mouse monoclonal antibody OPAL1-01.
11-699_WB
Western blotting analysis of human OPAL1 using mouse monoclonal antibody OPAL1-01 on lysates of HEK-293T/OPAL1 transfectants, and of murine splenocytes and HEK-293T cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-OPAL1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. OPAL1 was detected at around 45-60 kDa.

General references:

Holleman A, den Boer ML, Cheok MH, Kazemier KM, Pei D, Downing JR, Janka-Schaub GE, Göbel U, Graubner UB, Pui CH, Evans WE, Pieters R: Expression of the outcome predictor in acute leukemia 1 (OPAL1) gene is not an independent prognostic factor in patients treated according to COALL or St Jude protocols. Blood. 2006 Sep 15;108(6):1984-90.
PubMed

Product specific references:

Borna S, Drobek A, Kralova J, Glatzova D, Splichalova I, Fabisik M, Pokorna J, Skopcova T, Angelisova P, Kanderova V, Starkova J, Stanek P, Matveichuk OV, Pavliuchenko N, Kwiatkowska K, Protty MB, Tomlinson MG, Alberich-Jorda M, Korinek V, Brdicka T: Transmembrane adaptor protein WBP1L regulates CXCR4 signalling and murine haematopoiesis. J Cell Mol Med. 2020 Jan;24(2):1980-1992.
PubMed
Variant
0.1 mg
11-699-C100
Ask for delivery term HERE
198.00 USD

0.025 mg
11-699-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
11-699-C100
Ask for delivery term HERE
198.00 USD

0.025 mg
11-699-C025
Delivery 1 week
99.00 USD

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