Exbio — Research products — Antibodies — Other intracellular antigens — Anti-OPAL1 PE

Anti-OPAL1 PE

Regulatory status

RUO

Antigen

OPAL1

Clone

OPAL1-01

Format

Reactivity

Human

Application

FC-IC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-699-C100

Ask for delivery term HERE

248.60 USD

0.025 mg

1P-699-C025

Delivery 1 week

124.30 USD

Variant

0.1 mg

1P-699-C100

Ask for delivery term HERE

248.60 USD

0.025 mg

1P-699-C025

Delivery 1 week

124.30 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody OPAL1-01 recognizes an intracellular epitope of OPAL1 (outcome predictor in acute leukemia 1), a transmembrane adaptor protein expressed mainly by megacaryocytes and platelets.

Application

FC-IC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Reactivity

Human

Immunogen

Recombinant fragment of human OPAL1 (amino acids 152-342)

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

WBP1L

Antigen description

OPAL1 (outcome predictor in acute leukemia 1) is an almost uncharacterized transmembrane adaptor protein expressed mainly by megacaryocytes and platelets. Its expression is enhanced on TEL/AML leukemic cells. The function of OPAL1 is unknown, although the presence of a cytochrome c-like heme-binding site and a transmembrane domain suggested OPAL1 may be involved in the mitochondrial electron transport chain. Its originally reported prognostic impact appears to be treatment dependent.

Entrez Gene ID 54838

UniProt ID Q9NX94

Separation of Hek-293 / OPAL1 transfected cells (red-filled) from untransfected Hek-293 (black-dashed) in flow cytometry analysis (intracellular staining) of cell suspension using anti-human OPAL1 (OPAL1-01) PE antibody 5,0 μg/ml.

Flow cytometry analysis of OPAL1 in human peripheral blood using mouse monoclonal antibody anti-OPAL1 (OPAL1-01) PE antibody (red). Blank sample is shown blue.

General references:

Holleman A, den Boer ML, Cheok MH, Kazemier KM, Pei D, Downing JR, Janka-Schaub GE, Göbel U, Graubner UB, Pui CH, Evans WE, Pieters R: Expression of the outcome predictor in acute leukemia 1 (OPAL1) gene is not an independent prognostic factor in patients treated according to COALL or St Jude protocols. Blood. 2006 Sep 15;108(6):1984-90.
PubMed

Product specific references:

Borna S, Drobek A, Kralova J, Glatzova D, Splichalova I, Fabisik M, Pokorna J, Skopcova T, Angelisova P, Kanderova V, Starkova J, Stanek P, Matveichuk OV, Pavliuchenko N, Kwiatkowska K, Protty MB, Tomlinson MG, Alberich-Jorda M, Korinek V, Brdicka T: Transmembrane adaptor protein WBP1L regulates CXCR4 signalling and murine haematopoiesis. J Cell Mol Med. 2020 Jan;24(2):1980-1992.
PubMed