Exbio — Research products — Antibodies — Other intracellular antigens — Anti-OPAL1 Alexa Fluor<sup>®</sup> 647

Anti-OPAL1 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

OPAL1

Clone

OPAL1-01

Format

Alexa Fluor^® 647

Reactivity

Human

Application

WB, FC (QC tested)

Excitation laser

red (633 nm)

Variant

0.1 mg

A6-699-C100

In stock

242.00 USD

Variant

0.1 mg

A6-699-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody OPAL1-01 recognizes an intracellular epitope of OPAL1 (outcome predictor in acute leukemia 1), a transmembrane adaptor protein expressed mainly by megacaryocytes and platelets.

Application

WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 0.5-4 µg/ml. Intracellular staining.

Reactivity

Human

Immunogen

Recombinant fragment of human OPAL1 (amino acids 152-342)

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

WBP1L

Antigen description

OPAL1 (outcome predictor in acute leukemia 1) is an almost uncharacterized transmembrane adaptor protein expressed mainly by megacaryocytes and platelets. Its expression is enhanced on TEL/AML leukemic cells. The function of OPAL1 is unknown, although the presence of a cytochrome c-like heme-binding site and a transmembrane domain suggested OPAL1 may be involved in the mitochondrial electron transport chain. Its originally reported prognostic impact appears to be treatment dependent.

Entrez Gene ID 54838

UniProt ID Q9NX94

Separation of HEK-293 cells stained using anti-human OPAL1 (OPAL1-01) Alexa Fluor® 647 antibody (concentration in sample 0.6 μg/ml, red-filled) from HEK-293 cells stained using mouse IgG2a isotype control (MOPC-173) Alexa Fluor® 647 antibody (concentration in sample 0.6 μg/ml, same as OPAL1 Alexa Fluor® 647 concentration, black-dashed) in flow cytometry analysis (intracellular staining) of HEK-293 cell suspension.

General references:

Holleman A, den Boer ML, Cheok MH, Kazemier KM, Pei D, Downing JR, Janka-Schaub GE, Göbel U, Graubner UB, Pui CH, Evans WE, Pieters R: Expression of the outcome predictor in acute leukemia 1 (OPAL1) gene is not an independent prognostic factor in patients treated according to COALL or St Jude protocols. Blood. 2006 Sep 15;108(6):1984-90.
PubMed

Product specific references:

Borna S, Drobek A, Kralova J, Glatzova D, Splichalova I, Fabisik M, Pokorna J, Skopcova T, Angelisova P, Kanderova V, Starkova J, Stanek P, Matveichuk OV, Pavliuchenko N, Kwiatkowska K, Protty MB, Tomlinson MG, Alberich-Jorda M, Korinek V, Brdicka T: Transmembrane adaptor protein WBP1L regulates CXCR4 signalling and murine haematopoiesis. J Cell Mol Med. 2020 Jan;24(2):1980-1992.
PubMed