Anti-NTAL Purified

Anti-NTAL Purified
Regulatory status
RUO
Antigen
NTAL
Clone
PAb (485)
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-485-C100
Ask for delivery term HERE
180.00 USD

0.025 mg
11-485-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody recognizes a defined intracellular region (aa 91-243) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
Application
Application details
Western blotting: Recommended dilution: 1 μg/ml; positive control: RAMOS human lymphoma cell line, THP-1 human monocytic leukemia cell line; negative control: JURKAT human T cell leukemia cell line; non-reducing conditions, 15% separating SDS-PAGE gel.
Immunohistochemistry (paraffin sections): Recommended dilution:10 μg/ml; positive tissue: spleen.
Reactivity
Human
Negative species
Mouse
Immunogen
Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
Concentration
1 mg/ml
Preparation
Purified from rabbit serum by affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LAB, LAT2
Antigen description
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Entrez Gene ID 7462
UniProt ID Q9GZY6
11-485 WB
Western blotting analysis (non-reducing conditions) of NTAL-transfected HEK-293 FT cells using polyclonal anti-human NTAL antibody. Lane 1: cell lysate of transfected HEK-293 FT cells Lane 2: cell lysate of non-transfected HEK-293 FT cells (negative control) (HEK-293 FT is a subclone of the primary human embryonal kidney epithelial cell line.)
11-485_IHC
Immunohistochemistry staining of human spleen (paraffin sections) with anti-NTAL polyclonal. Commercially tested by LifeSpan BioSciences.

Product specific references:

Tkaczyk C, Horejsi V, Iwaki S, Draber P, Samelson LE, Satterthwaite AB, Nahm DH, Metcalfe DD, Gilfillan AM: NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood. 2004 Jul 1;104(1):207-14.
PubMed
Variant
0.1 mg
11-485-C100
Ask for delivery term HERE
180.00 USD

0.025 mg
11-485-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD