Anti-NTAL Purified

Anti-NTAL Purified
Regulatory status
RUO
Antigen
NTAL
Clone
PAb (487)
Format
Purified
Reactivity
Rat, Mouse
Application
WB, ICC
Variant
0.1 mg
11-487-C100
In stock

0.025 mg
11-487-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody reacts with Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 25 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
Application
WB, ICC
Reactivity
Rat, Mouse
Negative species
Human
Immunogen
Recombinant mouse NTAL (aa 30-203).
Other names
LAB, LAT2
Concentration
1 mg/ml
Preparation
Purified from rabbit serum by affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-487 WB
Western blotting analysis (non-reducing conditions) of mouse NTAL in mouse spleen tissue. Lane 1: immunostaining with polyclonal anti-NTAL antibody Lane 2: immunostaining with polyclonal anti-mouse NTAL antibody (specific for N-terminal domain of mouse NTAL; cat. no. 11-486-C100)
11-487 ICC
Immunocytochemistry detection (electron microscopy) of distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC; A) and RBL rat basophilic leukemia cell line (B) by anti-LAT (LAT-1D1; cat. no. 11-442-C100) and polyclonal anti-NTAL antibody, followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively.

Product specific references:

Lebduška P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328(1-2):139-51.
PubMed
Heneberg P, Lebduška P, Draberova L, Korb J, Draber P.: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806.
PubMed
Volná P, Lebduška P, Dráberová L, Símová S, Heneberg P, Boubelík M, Bugajev V, Malissen B, Wilson BS, Horejsí V, Malissen M, Dráber P.: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200(8):1001-13.
PubMed
Variant
0.1 mg
11-487-C100
In stock

0.025 mg
11-487-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock