Anti-NTAL Purified

Anti-NTAL Purified
Regulatory status
RUO
Antigen
NTAL
Clone
NAP-07
Format
Purified
Reactivity
Human, Mouse
Application
Variant
0.1 mg
11-374-C100
In stock
180.00 USD

0.025 mg
11-374-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody NAP-07 reacts with an intracellular epitope of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B lymphocytes, NK cells and myeloid cells.
Application
Application details
Western blotting: Recommended dilution: 2 μg/ml, incubation 60 min; positive control: RAMOS human lymphoma cell line, negative control: HeLa human cervix carcinoma cell line; non-reducing conditions, 12% separating SDS-PAGE gel.
Flow cytometry: Intracellular staining; recommended dilution: 1-4 µg/ml; positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.
Reactivity
Human, Mouse
Immunogen
Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LAB, LAT2
Antigen description
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Entrez Gene ID 56743
UniProt ID Q9JHL0
11-374_ICC
Immunocytochemistry analysis of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. The cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).

General references:

Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
PubMed
Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15.
PubMed

Product specific references:

Lebduška P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328(1-2):139-51.
PubMed
Volná P, Lebduška P, Dráberová L, Šímová S, Heneberg P, Boubelík M, Bugajev V, Malissen B, Wilson BS, Hořejší V, Malissen M, Dráber P: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200(8):1001-13.
PubMed
Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V.: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16; 196(12):1617-26.
PubMed
Variant
0.1 mg
11-374-C100
In stock
180.00 USD

0.025 mg
11-374-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD

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