Exbio — Research products — Antibodies — Other intracellular antigens — Anti-NTAL Purified

Anti-NTAL Purified

Regulatory status

RUO

Antigen

NTAL

Clone

NAP-03

Format

Purified

Reactivity

Human

Application

IP, WB, IHC(P)

Variant

0.1 mg

11-373-C100

In stock

198.00 USD

0.025 mg

11-373-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-373-C100

In stock

198.00 USD

0.025 mg

11-373-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody NAP-03 recognizes a defined intracellular epitope (aa 196-212) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B cells, NK cells and myeloid cells.

Application

IP, WB, IHC(P)

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: tonsil, germinal center.
Western blotting: Recommended dilution: 1-2 μg/ml, incubation 60 min; positive control: RAMOS human lymphoma cell line; non-reducing conditions, 12% separating SDS-PAGE gel.

Reactivity

Human

Negative species

Mouse

Immunogen

Peptide corresponding to aa 196-212 of human NTAL coupled to KLH.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LAB, LAT2

Antigen description

NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

Entrez Gene ID 7462

UniProt ID Q9GZY6

Product specific references:

Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196(12):1617-26.
PubMed