Exbio — Research products — Antibodies — Other intracellular antigens — Anti-NTAL Purified

Anti-NTAL Purified

Regulatory status

RUO

Antigen

NTAL

Clone

NAP-07

Format

Purified

Reactivity

Human, Mouse

Application

FC(IC), IP, WB, ICC

Variant

0.1 mg

11-374-C100

In stock

198.00 USD

0.025 mg

11-374-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-374-C100

In stock

198.00 USD

0.025 mg

11-374-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody NAP-07 reacts with an intracellular epitope of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B lymphocytes, NK cells and myeloid cells.

Application

FC(IC), IP, WB, ICC

Application details

Western blotting: Recommended dilution: 1-2 μg/ml.
Flow cytometry: Intracellular staining; recommended dilution: 1-4 µg/ml; positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.

Reactivity

Human, Mouse

Immunogen

Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LAB, LAT2

Antigen description

NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

Entrez Gene ID 56743

UniProt ID Q9JHL0

Immunocytochemistry analysis of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. The cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 negative NTAL positive lymphocytes (red-filled) from CD3 positive NTAL negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 μg/ml, GAM APC).

Western blotting analysis of human NTAL using mouse monoclonal antibody NAP-07 on lysates of Raji and Ramos cells and on Jurkat cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. NTAL was detected around 27 kDa.

General references:

Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
PubMed

Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15.
PubMed

Product specific references:

Lebduška P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328(1-2):139-51.
PubMed

Volná P, Lebduška P, Dráberová L, Šímová S, Heneberg P, Boubelík M, Bugajev V, Malissen B, Wilson BS, Hořejší V, Malissen M, Dráber P: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200(8):1001-13.
PubMed

Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V.: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16; 196(12):1617-26.
PubMed