Anti-Nitrotyrosine Purified

Anti-Nitrotyrosine Purified
Regulatory status
RUO
Antigen
Nitrotyrosine
Clone
EM-30
Format
Purified
Reactivity
Species independent
Variant
0.1 mg
11-693-C100
In stock
260.00 USD

0.025 mg
11-693-C025
Delivery 1 week
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
260.00 USD

0.1 mg
11-261-C100
In stock
130.00 USD
Product details
Description
Images
Isotype
Mouse IgG1
Specificity
The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
Western blotting: positive control: NO2-BSA, NO2-HSA.
Reactivity
Species independent
Immunogen
NO2-Tyr-CH2-Thyroglobulin
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TyrNO<sub>2</sub>, C<sub>9</sub>H<sub>10</sub>N<sub>2</sub>O<sub>5</sub>
Antigen description
Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.
11-693_IHC
Immunohistochemistry staining (frozen sections) of human tumoral colon epithelium with anti-nitrotyrosine antibody (EM-30).
Variant
0.1 mg
11-693-C100
In stock
260.00 USD

0.025 mg
11-693-C025
Delivery 1 week
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
260.00 USD

0.1 mg
11-261-C100
In stock
130.00 USD