Anti-NCK1 Purified

Anti-NCK1 Purified
Regulatory status
RUO
Antigen
NCK1
Clone
EM-06
Format
Purified
Reactivity
Mouse, Human
Application
Variant
0.1 mg
11-711-C100
In stock
180.00 USD

0.025 mg
11-711-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.
Application
Application details
Western blotting: Recommended dilution: 2 μg/ml; positive control: Jurkat cell lysate.
Immunocytochemistry: Recommended dilution: 2 μg/ml; positive control: human NCK1-transfected COS-7 cells.
Reactivity
Mouse, Human
Immunogen
Purified recombinant second SH3 domain of human Nck1 fused to GST
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
NCK-1, NCKalpha
Antigen description
NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.
Entrez Gene ID 4690
UniProt ID P16333
11-711 WB
Western blotting analysis of NCK1 in whole cell lysate of mouse lymph node lymphocytes using the antibody EM-06.

Product specific references:

Borroto A, Arellano I, Dopfer EP, Prouza M, Suchánek M, Fuentes M, Orfao A, Schamel WW, Alarcón B: Nck recruitment to the TCR required for ZAP70 activation during thymic development. J Immunol. 2013 Feb 1;190(3):1103-12.
PubMed
Variant
0.1 mg
11-711-C100
In stock
180.00 USD

0.025 mg
11-711-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD