Exbio — Research products — Antibodies — Other intracellular antigens — Anti-Ms Lime1 Purified

Anti-Ms Lime1 Purified

Anti-Ms Lime1 Purified
Regulatory status
RUO
Antigen
LIME1
Clone
mLIME-05
Format
Purified
Reactivity
Mouse
Application
IP, WB
Variant
0.1 mg
11-620-C100
Ask for delivery term HERE
180.00 USD
0.025 mg
11-620-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-620-C100
Ask for delivery term HERE
180.00 USD
0.025 mg
11-620-C025
Delivery 1 week
90.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG2a
Specificity
Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.
Application
IP, WB
Application details
Immunoprecipitation: Positive control: murine splenic T cells.
Western blotting: Reducing conditions, recommended dilution: 1-5 μg/ml.
Reactivity
Mouse
Immunogen
Recombinant protein corresponding to amino acids 86-202 of mouse LIME
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Lita1
Antigen description
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Entrez Gene ID 72699
UniProt ID Q9EQR5
11-620 WB
Western blotting analysis of murine thymocyte lysate (1% SDS); non-reduced sample, immunostained by mAb mLIME-05 and goat anti-mouse IgG (H+L)-HRP conjugate.

General references:

Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed
Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49.
PubMed
Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
PubMed
Variant
0.1 mg
11-620-C100
Ask for delivery term HERE
180.00 USD
0.025 mg
11-620-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-620-C100
Ask for delivery term HERE
180.00 USD
0.025 mg
11-620-C025
Delivery 1 week
90.00 USD
Contact distributor
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