Exbio — Research products — Antibodies — Other intracellular antigens — Anti-Ms Bim Purified

Anti-Ms Bim Purified

Anti-Ms Bim Purified
Regulatory status
RUO
Antigen
Bim
Clone
Ham151-149
Format
Purified
Reactivity
Mouse
Application
FC, IP, WB, ICC
Variant
0.1 mg
11-569-C100
Out of stock
240.00 USD
0.025 mg
11-569-C025
Out of stock
120.00 USD
Variant
0.1 mg
11-261-C100
In stock
240.00 USD
0.1 mg
11-261-C100
In stock
120.00 USD
Contact distributor
Product details
References
Isotype
Armenian Hamster IgG1 lambda
Specificity
The Armenian hamster antibody Ham151-149 reacts with mouse Bim, a 19-24 kDa pro-apoptotic intracellular protein (Bcl-2 family) which regulates immunological responses.
Application
FC, IP, WB, ICC
Application details
Flow cytometry: Intracellular staining; recommended dilution: 8-10 μg / ml; cells permeabilized with 0.03% saponin.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.
Western blotting: Recommended dilution: 1-2 μg / ml; reducing conditions.
Immunocytochemistry: permeabilized cells.
Reactivity
Mouse
Immunogen
Mouse BimL (w/o transmembrane region) + Bcl-xL (w/o transmembrane region)-6-his tag, purified from Hi5 cells
Other names
BAM, BOD
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

General references:

Wang X, Xing D, Liu L, Chen WR: BimL directly neutralizes Bcl-xL to promote Bax activation during UV-induced apoptosis. FEBS Lett. 2009 May 8. [Epub ahead of print]
PubMed
Yang Y, Zhao Y, Liao W, Yang J, Wu L, Zheng Z, Yu Y, Zhou W, Li L, Feng J, Wang H, Zhu WG: Acetylation of FoxO1 activates Bim expression to induce apoptosis in response to histone deacetylase inhibitor depsipeptide treatment. Neoplasia. 2009 Apr;11(4):313-24.
PubMed
Ludwinski MW, Sun J, Hilliard B, Gong S, Xue F, Carmody RJ, Devirgiliis J, Chen YH: Critical roles of Bim in T cell activation and T cell-mediated autoimmune inflammation in mice. J Clin Invest. 2009 May 1. pii: 37619. doi: 10.1172/JCI37619. [Epub ahead of print]
PubMed
Snow AL, Oliveira JB, Zheng L, Dale JK, Fleisher TA, Lenardo MJ: Critical role for BIM in T cell receptor restimulation-induced death. Biol Direct. 2008 Aug 20;3:34.
PubMed
Hughes P, Bouillet P, Strasser A: Role of Bim and other Bcl-2 family members in autoimmune and degenerative diseases. Curr Dir Autoimmun. 2006;9:74-94.
PubMed

Product specific references:

Oliver PM, Wang M, Zhu Y, White J, Kappler J, Marrack P: Loss of Bim allows precursor B cell survival but not precursor B cell differentiation in the absence of interleukin 7. J Exp Med. 2004 Nov 1;200(9):1179-87.
PubMed
Jorgensen TN, McKee A, Wang M, Kushnir E, White J, Refaeli Y, Kappler JW, Marrack P: Bim and Bcl-2 mutually affect the expression of the other in T cells. J Immunol. 2007 Sep 15;179(6):3417-24.
PubMed
Variant
0.1 mg
11-569-C100
Out of stock
240.00 USD
0.025 mg
11-569-C025
Out of stock
120.00 USD
Variant
0.1 mg
11-261-C100
In stock
240.00 USD
0.1 mg
11-261-C100
In stock
120.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product