Anti-LYN Purified

Anti-LYN Purified
Regulatory status
RUO
Antigen
LYN
Clone
LYN-01
Format
Purified
Reactivity
Rat, Human, Mouse
Application
Variant
0.1 mg
11-377-C100
In stock
180.00 USD

0.025 mg
11-377-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues (intracellular antigen).
Application
Application details
Western blotting: Recommended dilution: 1-2 μg/ml.
Reactivity
Rat, Human, Mouse
Immunogen
Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238).
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
JTK8, p56Lyn, p53Lyn
Antigen description
Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease.
Entrez Gene ID 4067
UniProt ID P07948
11-377_WB
Western blotting analysis of human LYN using mouse monoclonal antibody LYN-01 on lysates of Raji cell line and Jurkat cell line (LYN non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-LYN monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LYN was detected around 55 kDa.

General references:

Strzelecka-Kiliszek A, Kwiatkowska K, Sobota A: Lyn and Syk kinases are sequentially engaged in phagocytosis mediated by Fc gamma R. J Immunol. 2002 Dec 15;169(12):6787-94.
PubMed
Rivera J, Olivera A: A current understanding of Fc epsilon RI-dependent mast cell activation. Curr Allergy Asthma Rep. 2008 Mar;8(1):14-20.
PubMed
Davey AM, Krise KM, Sheets ED, Heikal AA: Molecular perspective of antigen-mediated mast cell signaling. J Biol Chem. 2008 Mar 14;283(11):7117-27.
PubMed
Kinoshita T, Miyano N, Nakai R, Yokota K, Ishiguro H, Tada T: Protein purification and preliminary crystallographic analysis of human Lyn tyrosine kinase. Protein Expr Purif. 2008 Apr;58(2):318-24.
PubMed
Dos Santos C, Demur C, Bardet V, Prade-Houdellier N, Payrastre B, Récher C: A critical role for Lyn in acute myeloid leukemia. Blood. 2008 Feb 15;111(4):2269-79.
PubMed

Product specific references:

Draberova L, Amoui M, Draber P.: Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains. Immunology. 1996 Jan;87(1):141-8.
PubMed
Amoui M, Draberova L, Tolar P, Drber P: Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors. Eur J Immunol. 1997 Jan;27(1):321-8.
PubMed
Kovarova M, Tolar P, Arudchandran R, Draberova L, Rivera J, Draber P: Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation. Mol Cell Biol. 2001 Dec;21(24):8318-28.
PubMed
Variant
0.1 mg
11-377-C100
In stock
180.00 USD

0.025 mg
11-377-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD