Exbio — Research products — Antibodies — Other intracellular antigens — Anti-LST1 Purified

Anti-LST1 Purified

Anti-LST1 Purified
Regulatory status
RUO
Antigen
LST1
Clone
LST1/02
Format
Purified
Reactivity
Human
Application
IP, WB, ICC
Variant
0.1 mg
11-528-C100
In stock
180.00 USD
0.025 mg
11-528-C025
In stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
Application
IP, WB, ICC
Application details
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.
Western blotting: The antigen migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
Reactivity
Human
Immunogen
KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1
Other names
LST-1, B144
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-528 WB
Western blotting analysis (non-reducing conditions) of LST1 in whole cell lysate of U937 human Caucasian histiocytic lymphoma cell line. Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100) Lane 2-3: immunostaining with anti-human LST1 (LST1/02), two independent batches. Note: It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

General references:

Rollinger-Holzinger I, Eibl B, Pauly M, Griesser U, Hentges F, Auer B, Pall G, Schratzberger P, Niederwieser D, Weiss EH, Zwierzina H: LST1: a gene with extensive alternative splicing and immunomodulatory function. J Immunol. 2000 Mar 15;164(6):3169-76.
PubMed
Yu X, Weissman SM: Characterization of the promoter of human leukocyte-specific transcript 1. A small gene with a complex pattern of alternative transcripts. J Biol Chem. 2000 Nov 3;275(44):34597-608.
PubMed
Raghunathan A, Sivakamasundari R, Wolenski J, Poddar R, Weissman SM: Functional analysis of B144/LST1: a gene in the tumor necrosis factor cluster that induces formation of long filopodia in eukaryotic cells. Exp Cell Res. 2001 Aug 15;268(2):230-44.
PubMed
Mulcahy H, O'Rourke KP, Adams C, Molloy MG, O'Gara F: LST1 and NCR3 expression in autoimmune inflammation and in response to IFN-gamma, LPS and microbial infection. Immunogenetics. 2006 Jan;57(12):893-903.
PubMed

Product specific references:

Draber P, Stepanek O, Hrdinka M, Drobek A, Chmatal L, Mala L, Ormsby T, Angelisova P, Horejsi V, Brdicka T: LST1/A is a myeloid leukocyte-specific transmembrane adaptor protein recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to the plasma membrane. J Biol Chem. 2012 Jun 29;287(27):22812-21
PubMed
Variant
0.1 mg
11-528-C100
In stock
180.00 USD
0.025 mg
11-528-C025
In stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
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