Exbio — Research products — Antibodies — Other intracellular antigens — Anti-LRRC32 Purified

Anti-LRRC32 Purified

Regulatory status

RUO

Antigen

LRRC32

Clone

GARP5

Format

Purified

Reactivity

Human

Application

Variant

0.1 mg

11-744-C100

In stock

143.00 USD

0.025 mg

11-744-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-744-C100

In stock

143.00 USD

0.025 mg

11-744-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody GARP5 recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.

Application

Application details

Western blotting: Recommended dilution: 1-2 μg/ml; positive control: human thrombocytes, reducing conditions

Reactivity

Human

Immunogen

Purified human sGARP protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

GARP, D11S833E

Antigen description

GARP (glycoprotein A repetitions predominant protein), also known as garpin or LRRC32 (leucin-rich repeat containing protein 32) is an approximately 80 kDa transmembrane glycoprotein detected on the surface of megakaryocytes, platelets, and activated Treg cells. It binds to the latency-associated peptide (LAP) domain of pro-TGF beta and regulates its storage and activation. The expression of GARP on Treg cells seems to be necessary for their suppressive functions.

Entrez Gene ID 2615

UniProt ID Q14392

Western blotting analysis (reducing conditions) of GARP antigen and its degradatory products in lysate of human thrombocytes by anti-GARP monoclonal antibody (GARP5).

Product specific references:

Wang R, Zhu J, Dong X, Shi M, Lu C, Springer TA: GARP regulates the bioavailability and activation of TGFβ. Mol Biol Cell. 2012 Mar;23(6):1129-39
PubMed