Anti-LIME1 Purified

Anti-LIME1 Purified
Regulatory status
RUO
Antigen
LIME1
Clone
LIME-10
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-401-C100
Ask for delivery term HERE
198.00 USD

0.025 mg
11-401-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
11-401-C100
Ask for delivery term HERE
198.00 USD

0.025 mg
11-401-C025
Delivery 1 week
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
Application
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml.
Western blotting: Reducing conditions; recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-5 μg/ml.
Reactivity
Human
Immunogen
COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LITA1
Antigen description
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Entrez Gene ID 54923
UniProt ID Q9H400
11-401_WB2
Western blotting analysis of human LIME using mouse monoclonal antibody LIME-10 on lysates of J77-LIME transfectants and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 1 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LIME was detected around 30 kDa.
11-401_FC_Histogram
Anti-Hu LIME1 purified antibody (clone LIME-10) specificity verification by flow cytometry

Analysis of the antibody staining was performed on fixed and permeabilized (eBioscience™ Intracellular Fixation & Permeabilization Buffer Set, Thermofisher) cell suspension.
Anti-Hu LIME1 purified antibody (clone LIME-10) (concentration in sample 1 μg/ml, red-filled histogram) binds specifically to intacellular LIME1 in transfected cells (upper panel), but not to Jurkat cells (lower panel).
Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 1 μg/ml, black-dashed histogram).

General references:

Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
PubMed
Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49.
PubMed

Product specific references:

Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed
Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
PubMed
Variant
0.1 mg
11-401-C100
Ask for delivery term HERE
198.00 USD

0.025 mg
11-401-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
11-401-C100
Ask for delivery term HERE
198.00 USD

0.025 mg
11-401-C025
Delivery 1 week
99.00 USD