Anti-LCK Alexa Fluor® 647

Anti-LCK Alexa Fluor<sup>®</sup> 647
Regulatory status
RUO
Antigen
LCK
Clone
LCK-01
Format
Alexa Fluor® 647
Reactivity
Human
Excitation laser
red (633 nm)
Variant
0.1 mg
A6-269-C100
In stock
320.00 USD

0.025 mg
A6-269-C025
Delivery 1 week
160.00 USD
Variant
0.1 mg
11-261-C100
In stock
320.00 USD

0.1 mg
11-261-C100
In stock
160.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase (intracellular antigen).
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Human
Negative species
Mouse
Immunogen
Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
Concentration
1 mg/ml
Preparation
The purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
p56Lck
Antigen description
Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.
Entrez Gene ID 3932
UniProt ID P06239
A6-269_FC_Profil Značení
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human LCK (LCK-01) Alexa Fluor® 647 antibody (concentration in sample 8 μg/ml).
A6-269_FC_Histogram
Separation of human LCK positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human LCK (LCK-01) Alexa Fluor® 647 antibody (concentration in sample 8 μg/ml).

General references:

Denny MF, Kaufman HC, Chan AC, Straus DB: The lck SH3 domain is required for activation of the mitogen-activated protein kinase pathway but not the initiation of T-cell antigen receptor signaling. J Biol Chem. 1999 Feb 19;274(8):5146-52.
PubMed
Gruber C, Henkel M, Budach W, Belka C, Jendrossek V: Involvement of tyrosine kinase p56/Lck in apoptosis induction by anticancer drugs. Biochem Pharmacol. 2004 May 15;67(10):1859-72.
PubMed
Hur YG, Yun Y, Won J: Rosmarinic acid induces p56lck-dependent apoptosis in Jurkat and peripheral T cells via mitochondrial pathway independent from Fas/Fas ligand interaction. J Immunol. 2004 Jan 1;172(1):79-87.
PubMed
Lovatt M, Filby A, Parravicini V, Werlen G, Palmer E, Zamoyska R: Lck regulates the threshold of activation in primary T cells, while both Lck and Fyn contribute to the magnitude of the extracellular signal-related kinase response. Mol Cell Biol. 2006 Nov;26(22):8655-65.
PubMed
Kim MJ, Park MT, Yoon CH, Byun JY, Lee SJ: Activation of Lck is critically required for sphingosine-induced conformational activation of Bak and mitochondrial cell death. Biochem Biophys Res Commun. 2008 May 30;370(2):353-8.
PubMed
Li M, Ong SS, Rajwa B, Thieu VT, Geahlen RL, Harrison ML: The SH3 domain of Lck modulates T-cell receptor-dependent activation of extracellular signal-regulated kinase through activation of Raf-1. Mol Cell Biol. 2008 Jan;28(2):630-41.
PubMed

Product specific references:

Cebecauer M, Cerny J, Horejsi V.: Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun. 1998 Feb 24;243(3):706-10.
PubMed
Romagnoli P, Strahan D, Pelosi M, Cantagrel A, van Meerwijk JP.: A potential role for protein tyrosine kinase p56(lck) in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness. Int Immunol. 2001 Mar;13(3):305-12.
PubMed
Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178(4):2262-71.
PubMed
Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
PubMed
Variant
0.1 mg
A6-269-C100
In stock
320.00 USD

0.025 mg
A6-269-C025
Delivery 1 week
160.00 USD
Variant
0.1 mg
11-261-C100
In stock
320.00 USD

0.1 mg
11-261-C100
In stock
160.00 USD

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