Exbio — Research products — Antibodies — Other intracellular antigens — Anti-LAT Purified

Anti-LAT Purified

Regulatory status

RUO

Antigen

LAT

Clone

LAT-01

Format

Purified

Reactivity

Human

Application

FC-IC, IP, WB

Variant

0.1 mg

11-360-C100

In stock

176.00 USD

0.025 mg

11-360-C025

Delivery 1 week

88.00 USD

Variant

0.1 mg

11-360-C100

In stock

176.00 USD

0.025 mg

11-360-C025

Delivery 1 week

88.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody LAT-01 reacts with an intracellular epitope of LAT, a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.

Application

FC-IC, IP, WB

Application details

Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized.
Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Human

Negative species

Mouse

Immunogen

Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IMD52

Antigen description

LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.

Entrez Gene ID 27040

UniProt ID O43561

Western blotting analysis of human LAT using mouse monoclonal antibody LAT-01 in lysates of Jurkat cells (positive) and Raji cells (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-LAT monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for LAT at approximately 38 kDa.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 positive LAT positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LAT (LAT-01) purified antibody (concentration in sample 1 μg/ml, GAM APC).

General references:

Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15.
PubMed

Product specific references:

Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V.: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed

Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem. 2011 Jun 3;286(22):19617-29.
PubMed