Exbio — Research products — Antibodies — Other intracellular antigens — Anti-LARGE1 Purified

Anti-LARGE1 Purified

Anti-LARGE1 Purified
Regulatory status
RUO
Antigen
LARGE1
Clone
LARGE-02
Format
Purified
Reactivity
Human
Application
WB, FC (QC tested)
Variant
0.1 mg
11-702-C100
In stock
180.00 USD
0.025 mg
11-702-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
Product details
Description
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase expressed mainly in the Golgi apparatus. Crossreactivity with LARGE2 was not determined.
Application
WB, FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Human
Immunogen
Recombinant fragment of human LARGE1 (amino acids 35-142)
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Xysosyl- and glucosyl transferase 1
Antigen description
LARGE1 serves as a glycosyltransferase which participates in glycosylation of the muscle membrane protein alpha-dystroglycan. Mutations of LARGE1 lead to hypoglycosylation of alpha-dystroglycan and cause congenital muscular dystrophy (MDC1D) associated with severe mental retardation. Altered alpha-dystroglycan glycosylation may also play a role in cancer, as hypoglycosylation of the protein and loss of laminin binding have been demonstrated in invasive carcinoma cells.
Entrez Gene ID 9215
UniProt ID O95461

General references:

Grewal PK, McLaughlan JM, Moore CJ, Browning CA, Hewitt JE: Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies. Glycobiology. 2005 Oct;15(10):912-23.
PubMed
Variant
0.1 mg
11-702-C100
In stock
180.00 USD
0.025 mg
11-702-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product