Anti-LARGE1 PE

Anti-LARGE1 PE
Regulatory status
RUO
Antigen
LARGE1
Clone
LARGE-02
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-702-C100
Ask for delivery term HERE
220.00 USD

0.025 mg
1P-702-C025
Delivery 1 week
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase expressed mainly in the Golgi apparatus. Crossreactivity with LARGE2 was not determined.
Application
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Human
Immunogen
Recombinant fragment of human LARGE1 (amino acids 35-142)
Concentration
0.1 mg/ml
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Xysosyl- and glucosyl transferase 1
Antigen description
LARGE1 serves as a glycosyltransferase which participates in glycosylation of the muscle membrane protein alpha-dystroglycan. Mutations of LARGE1 lead to hypoglycosylation of alpha-dystroglycan and cause congenital muscular dystrophy (MDC1D) associated with severe mental retardation. Altered alpha-dystroglycan glycosylation may also play a role in cancer, as hypoglycosylation of the protein and loss of laminin binding have been demonstrated in invasive carcinoma cells.
Entrez Gene ID 9215
UniProt ID O95461
1P-702 FC
Flow cytometry analysis of LARGE1 in HEK293-LARGE1 transfectants using mouse monoclonal antibody (clone LARGE-02) PE.

General references:

Grewal PK, McLaughlan JM, Moore CJ, Browning CA, Hewitt JE: Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies. Glycobiology. 2005 Oct;15(10):912-23.
PubMed
Variant
0.1 mg
1P-702-C100
Ask for delivery term HERE
220.00 USD

0.025 mg
1P-702-C025
Delivery 1 week
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD