Exbio — Research products — Antibodies — Other intracellular antigens — Anti-Hu GARP Purified

Anti-Hu GARP Purified

Anti-Hu GARP Purified
Regulatory status
RUO
Antigen
GARP
Clone
GARP5
Format
Purified
Reactivity
Human
Application
FC, WB
Variant
0.1 mg
11-744-C100
In stock
147.40 USD
0.025 mg
11-744-C025
Delivery 1 week
73.70 USD
Variant
0.1 mg
11-744-C100
In stock
147.40 USD
0.025 mg
11-744-C025
Delivery 1 week
73.70 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody GARP5 recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.
Application
FC, WB
Application details
Western blotting: Recommended dilution: 1-2 μg/ml; positive control: human thrombocytes, reducing conditions
Reactivity
Human
Immunogen
Purified human sGARP protein
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LRRC32, garpin, D11S833E
Antigen description
GARP (glycoprotein A repetitions predominant protein), also known as garpin or LRRC32 (leucin-rich repeat containing protein 32) is an approximately 80 kDa transmembrane glycoprotein detected on the surface of megakaryocytes, platelets, and activated Treg cells. It binds to the latency-associated peptide (LAP) domain of pro-TGF beta and regulates its storage and activation. The expression of GARP on Treg cells seems to be necessary for their suppressive functions.
Entrez Gene ID 2615
UniProt ID Q14392
11-744_WB_appl
Anti-Hu GARP Purified (clone GARP5) works in WB application under reducing conditions.

Western blotting analysis was performed on RIPA buffer extracts of thrombocytes, leukocytes, and Jurkat cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody GARP5 (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml) was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.

GARP was detected at ~80 kDa in thrombocytes.

Product specific references:

Wang R, Zhu J, Dong X, Shi M, Lu C, Springer TA: GARP regulates the bioavailability and activation of TGFβ. Mol Biol Cell. 2012 Mar;23(6):1129-39
PubMed
Variant
0.1 mg
11-744-C100
In stock
147.40 USD
0.025 mg
11-744-C025
Delivery 1 week
73.70 USD
Variant
0.1 mg
11-744-C100
In stock
147.40 USD
0.025 mg
11-744-C025
Delivery 1 week
73.70 USD
Contact distributor
Datasheet download
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