Exbio — Research products — Antibodies — Other intracellular antigens — Anti-Hu BCL2 Purified

Anti-Hu BCL2 Purified

Regulatory status

RUO

Antigen

BCL2

Clone

Bcl-2/100

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC-F, IHC-P, ICC

Variant

0.1 mg

11-668-C100

In stock

165.00 USD

0.025 mg

11-668-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-668-C100

In stock

165.00 USD

0.025 mg

11-668-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa intracellular protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.

Application

IP, WB, FC (QC tested), IHC-F, IHC-P, ICC

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Western blotting: Recommended dilution: 1-2 µg/ml.

Reactivity

Human

Negative species

Mouse

Immunogen

Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Bcl-2, PPP1R50

Antigen description

Bcl2 (B cell chronic lymphatic leukemia protein 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.

Entrez Gene ID 596

UniProt ID P10415

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human BCL-2 (Bcl-2/100) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human BCL-2 (Bcl-2/100) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Immunohistochemistry staining of human placenta (paraffin-embedded sections) with anti-BCL2 (Bcl-2/100), 10 μg/ml. Commercially tested by LifeSpan BioSciences.

Western blotting analysis of human BCL2 using mouse monoclonal antibody Bcl-2/100 on lysates of Jurkat and Raji cells, and 3T3 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody.

Product specific references:

Joubert A, Marais S, Maritz C: Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels. Biocell. 2009 Apr;33(1):67-70.
PubMed

Laforge M, Petit F, Estaquier J, Senik A: Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef. J Virol. 2007 Oct;81(20):11426-40.
PubMed

Laflamme C, Israël-Assayag E, Cormier Y: Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis. Eur Respir J. 2003 Feb;21(2):225-31.
PubMed

Soilu-Hänninen M, Ekert P, Bucci T, Syroid D, Bartlett PF, Kilpatrick TJ: Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway. J Neurosci. 1999 Jun 15;19(12):4828-38.
PubMed

Gugasyan R, Christou A, O'Reilly LA, Strasser A, Gerondakis S: Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA. Cell Death Differ. 2006 Jul;13(7):1235-7.
PubMed