Anti-Hu BCL2 PE

Anti-Hu BCL2 PE
Regulatory status
RUO
Antigen
BCL2
Clone
Bcl-2/100
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-668-T100
In stock
286.00 USD

25 tests
1P-668-T025
Delivery 1 week
143.00 USD
Variant
100 tests
1P-668-T100
In stock
286.00 USD

25 tests
1P-668-T025
Delivery 1 week
143.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa intracellular protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Human
Negative species
Mouse
Immunogen
Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Bcl-2, PPP1R50
Antigen description
Bcl2 (B cell chronic lymphatic leukemia protein 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.
Entrez Gene ID 596
UniProt ID P10415
1P-668_FC_Profil Značení_DLBCL
Flow cytometry intracellular staining pattern of human peripheral whole blood from diffuse large B-cell lymphoma (DLBCL) patient. Sample was stained using anti-human BCL2 (Bcl-2/100) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-668_FC_Dot-plot_DLBCL
Flow cytometry multicolor staining of human lymphocytes stained using anti-human BCL2 (Bcl-2/100) PE antibody (intracellular staining, 10 μl reagent / 100 μl of peripheral whole blood from DLBCL patient) and anti-human CD19 (LT19) FITC antibody (surface staining, 20 μl reagent / 100 μl of peripheral whole blood from DLBCL patient).
1P-668_FC_Histogram_DLBCL
Separation of human BCL-2 positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood from DLBCL patient stained using anti-human BCL2 (Bcl-2/100) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-668 FC
Flow cytometry analysis (intracellular staining) of human peripheral blood with anti-Bcl2 (Bcl-2/100) PE.

Product specific references:

Joubert A, Marais S, Maritz C: Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels. Biocell. 2009 Apr;33(1):67-70.
PubMed
Laforge M, Petit F, Estaquier J, Senik A: Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef. J Virol. 2007 Oct;81(20):11426-40.
PubMed
Laflamme C, Israël-Assayag E, Cormier Y: Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis. Eur Respir J. 2003 Feb;21(2):225-31.
PubMed
Soilu-Hänninen M, Ekert P, Bucci T, Syroid D, Bartlett PF, Kilpatrick TJ: Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway. J Neurosci. 1999 Jun 15;19(12):4828-38.
PubMed
Gugasyan R, Christou A, O'Reilly LA, Strasser A, Gerondakis S: Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA. Cell Death Differ. 2006 Jul;13(7):1235-7.
PubMed
Variant
100 tests
1P-668-T100
In stock
286.00 USD

25 tests
1P-668-T025
Delivery 1 week
143.00 USD
Variant
100 tests
1P-668-T100
In stock
286.00 USD

25 tests
1P-668-T025
Delivery 1 week
143.00 USD

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